Werness P G, Bergert J H, Smith L H
J Lab Clin Med. 1982 Feb;99(2):254-62.
The commonly used diuretic Tri and its metabolites have been identified recently as major components of some kidney stones. We have carried out basic physical chemical studies to determine the mechanism of Tri incorporation into kidney stones. The solubility and pK of Tri and its major metabolites, Tri-OH and the Tri-So4, have been determined at 37 C in 0.15 M NaCl. The effect of Tri and its metabolites on crystal formation has been measured in the calcium oxalate monohydrate, hydroxyapatite, and uric acid crystal systems. Tri and its metabolites do not promote crystal nucleation, growth, or aggregation in any of these crystal systems and are not incorporated as these crystals form. We can demonstrate binding of Tri and its metabolites to the protein matrix isolated from kidney stones. These data suggest that Tri and its metabolites to the protein matrix isolated from kidney stones. These data suggest that Tri and its metabolites do not promote the formation of kidney stones but rather become incorporated into existing stones or stone nidi by binding to the protein matrix found in all kidney stones.
常用利尿剂曲安西龙及其代谢产物最近被确定为某些肾结石的主要成分。我们进行了基础物理化学研究，以确定曲安西龙掺入肾结石的机制。已在37℃下于0.15M氯化钠中测定了曲安西龙及其主要代谢产物曲安西龙 - OH和曲安西龙 - So4的溶解度和pK值。在一水合草酸钙、羟基磷灰石和尿酸晶体系统中测量了曲安西龙及其代谢产物对晶体形成的影响。曲安西龙及其代谢产物在任何这些晶体系统中均不促进晶体成核、生长或聚集，并且在这些晶体形成时不会掺入其中。我们可以证明曲安西龙及其代谢产物与从肾结石中分离出的蛋白质基质结合。这些数据表明曲安西龙及其代谢产物与从肾结石中分离出的蛋白质基质结合。这些数据表明曲安西龙及其代谢产物不会促进肾结石的形成，而是通过与所有肾结石中发现的蛋白质基质结合而掺入现有的结石或结石核心中。
