Department of Dermatology, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin, China.
Neoplasma. 2019 Jul 23;66(4):584-592. doi: 10.4149/neo_2018_181123N888. Epub 2019 Apr 24.
Neuroblastoma breakpoint family member 1 (NBPF1) is involved in the occurrence and development of tumors. However, only a limited number of studies were conducted on NBPF1 and cutaneous squamous cell carcinoma (SCC). This study mainly explored the expression and mechanism of NBPF1 in SCC. SCC tissue and adjacent tissues samples were randomly selected. NBPF1 gene was overexpressed in the A431 cell line using plasmid transfection technique. Cell viability was tested by cell counting kit-8 (CCK-8) assay. Flow cytometry was used to determine cell cycle and apoptosis. Western blot and RT-qPCR were respectively performed to determine the expression levels of proteins and mRNAs. The NBPF1 gene was lowly expressed in SCC tissues. The expression level of NBPF1 gene was the lowest in A431 cell line. The cell viability of A431 was reduced after transfection. Overexpression of NBPF1 not only arrested A431 cells in G1 phase and promoted apoptosis, but also up-regulated the expressions of Bax and p53 mRNA and protein and down-regulated the expressions of Bcl-2, Survivin and Cyclin D1. Akt-p53-Cyclin pathway was inhibited when NBPF1 gene expression was up-regulated. Upregulation of NBPF1 might promote apoptosis of A431 cells and block cell cycle via inhibiting the activation of Akt-p53-Cyclin signaling pathway.
神经母细胞瘤断点家族成员 1（NBPF1）参与肿瘤的发生和发展。然而，仅有少数研究针对 NBPF1 和皮肤鳞状细胞癌（SCC）进行了研究。本研究主要探讨了 NBPF1 在 SCC 中的表达和作用机制。随机选择 SCC 组织和相邻组织样本。通过质粒转染技术在 A431 细胞系中过表达 NBPF1 基因。通过细胞计数试剂盒-8（CCK-8）测定细胞活力。通过流式细胞术测定细胞周期和凋亡。分别通过 Western blot 和 RT-qPCR 测定蛋白和 mRNA 的表达水平。NBPF1 基因在 SCC 组织中低表达。NBPF1 基因在 A431 细胞系中的表达水平最低。转染后 A431 的细胞活力降低。过表达 NBPF1 不仅使 A431 细胞停滞在 G1 期并促进凋亡，而且上调了 Bax 和 p53 mRNA 和蛋白的表达，下调了 Bcl-2、Survivin 和 Cyclin D1 的表达。上调 NBPF1 可能通过抑制 Akt-p53-Cyclin 信号通路的激活来促进 A431 细胞凋亡并阻断细胞周期。
