从cDNA序列推导的食蟹猴载脂蛋白A-1的一级结构：与人类序列的比较。

The primary structure of cynomolgus monkey apolipoprotein A-1 deduced from the cDNA sequence: comparison to the human sequence.

作者信息

Polites H G, Melchior G W, Castle C K, Marotti K R

出版信息

Gene. 1986;49(1):103-10. doi: 10.1016/0378-1119(86)90389-6.

Abstract

We have cloned and analyzed a cDNA containing the complete coding sequence for cynomolgus monkey apolipoprotein A-1 (apoA-1). This cDNA clone was found to share approx. 97% nucleotide sequence identity with the published human apoA-1 and encodes a protein of the same size as the human protein. Paired proline residues are present at positions 3 and 4 in the mature protein as has been reported for other primate species and the propeptide sequence is identical to the human propeptide. The amino acid content derived from the nucleotide sequence predicts a more basic protein than human apoA-1 and this was confirmed by isoelectric focusing analysis. In addition, we present evidence for two different transcriptional initiation sites for the cynomolgus monkey gene in contrast to only one for human.

摘要

我们已经克隆并分析了一个包含食蟹猴载脂蛋白A-1（apoA-1）完整编码序列的cDNA。发现该cDNA克隆与已发表的人类apoA-1的核苷酸序列同一性约为97%，并编码一个与人类蛋白质大小相同的蛋白质。成熟蛋白的第3和第4位存在成对的脯氨酸残基，这与其他灵长类物种的报道一致，并且前肽序列与人类前肽相同。从核苷酸序列推导的氨基酸含量预测该蛋白比人类apoA-1更具碱性，等电聚焦分析证实了这一点。此外，我们提供证据表明食蟹猴基因有两个不同的转录起始位点，而人类只有一个。