Osada J, Pocoví M, Nicolosi R J, Schaefer E J, Ordovás J M
Department of Clinical Sciences, University of Lowell, MA.
Biochim Biophys Acta. 1993 Mar 20;1172(3):335-9. doi: 10.1016/0167-4781(93)90226-4.
The cynomolgus monkey (Macaca fascicularis) apolipoprotein C-III and apolipoprotein A-IV genes have been isolated from a cynomolgus genomic DNA library and completely sequenced. These genes span 3.1 and 2.8 kilobases (kb), respectively. Apolipoprotein C-III gene is interrupted by three intervening sequences of 613, 135 and 1699 bp, respectively. The open reading frame encodes a protein of 99 amino acids which is 87% similar to the human. The cynomolgus mature protein is 79 residues long. Thr-74 is also present what might allow the formation of O-glycosidic linkage observed in the human protein. Apolipoprotein A-IV gene consist of two intervening sequences of 352 and 774 bp, respectively. The open reading frame encodes a protein of 429 amino acids which is 87% similar to the human. The cynomolgus mature protein is 409 residues long, 33 amino acids longer than the human, due to an insertion of 33 residues in its COOH-terminal region. This insertion is mainly composed of glutamine and glutamic acid, which confers cynomolgus apolipoprotein a higher hydrophilicity.
食蟹猴（猕猴）载脂蛋白C-III和载脂蛋白A-IV基因已从食蟹猴基因组DNA文库中分离出来并进行了全序列测定。这些基因分别跨越3.1和2.8千碱基（kb）。载脂蛋白C-III基因分别被三个长度为613、135和1699 bp的间隔序列中断。开放阅读框编码一种由99个氨基酸组成的蛋白质，与人的该蛋白质有87%的相似性。食蟹猴的成熟蛋白长度为79个残基。苏氨酸-74也存在，这可能允许形成在人蛋白质中观察到的O-糖苷键。载脂蛋白A-IV基因分别由两个长度为352和774 bp的间隔序列组成。开放阅读框编码一种由429个氨基酸组成的蛋白质，与人的该蛋白质有87%的相似性。食蟹猴的成熟蛋白长度为409个残基，比人的长33个氨基酸，这是由于其COOH末端区域插入了33个残基。这种插入主要由谷氨酰胺和谷氨酸组成，这赋予食蟹猴载脂蛋白更高的亲水性。
