雪榕虫草交配型（MAT）基因座的克隆与分析。

Cloning and analysis of Ophiocordyceps xuefengensis mating type (MAT) loci.

机构信息

Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Science, Hunan Normal University, Changsha 410081, China.

Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China.

出版信息

FEMS Microbiol Lett. 2019 Apr 1;366(7). doi: 10.1093/femsle/fnz070.

DOI:10.1093/femsle/fnz070

PMID:31062026

Abstract

The entomopathogenic fungus Ophiocordyceps xuefengensis, a recently described species and identified as the sister taxon of Ophiocordyceps sinensis, is a desirable alternative to O. sinensis. The mating systems of fungi play a vitally important role in the regulation of sexual reproduction and evolution, but the mating type loci of O. xuefengensis were completely unknown. In this study, the mating systems of O. xuefengensis were analyzed. The conserved α-box region of the MAT1-1-1 and HMG-box of MAT1-2-1 were successfully obtained by PCR amplification. The distribution of both mating types in different tissues of wild and cultivated O. xuefengensis growth was detected and analyzed. The results showed that the asci always harbored both mating types, whereas the sclerotium, the stipe and each isolated strain of wild O. xuefengensis always had only one idiomorph, either MAT1-1 or MAT1-2, which confirmed that O. xuefengensis is heterothallic. The MAT1-1 locus of O. xuefengensis harbors MAT1-1-1, MAT1-1-2 and MAT1-1-3, and MAT1-2 contains the MAT1-2-1 gene. Southern blot analysis showed the MAT-1-1-1 and MAT-1-2-1 genes were single-copy in O. xuefengensis. These results will help to understand its life cycle and support artificial cultivation of O. xuefengensis.

摘要

虫生真菌细脚拟青霉是最近描述的一个物种，被鉴定为中华被毛孢的姐妹种，是中华被毛孢的理想替代品。真菌的交配系统在调节有性生殖和进化方面起着至关重要的作用，但细脚拟青霉的交配型基因座完全未知。本研究分析了细脚拟青霉的交配系统。通过 PCR 扩增成功获得了 MAT1-1-1 的保守α-盒区和 MAT1-2-1 的 HMG 盒。检测并分析了野生和栽培细脚拟青霉生长过程中不同组织中两种交配型的分布。结果表明，子囊总是含有两种交配型，而菌核、菌柄和野生细脚拟青霉的每个分离菌株只含有一种单倍型，即 MAT1-1 或 MAT1-2，这证实了细脚拟青霉是异宗配合的。细脚拟青霉的 MAT1-1 基因座含有 MAT1-1-1、MAT1-1-2 和 MAT1-1-3，MAT1-2 包含 MAT1-2-1 基因。Southern blot 分析表明，MAT-1-1-1 和 MAT-1-2-1 基因在细脚拟青霉中是单拷贝的。这些结果将有助于了解其生命周期，并支持细脚拟青霉的人工栽培。