质体别藻蓝蛋白基因在蓝细菌中的功能表达
Functional expression of plastid allophycocyanin genes in a cyanobacterium.
作者信息
de Lorimier R, Guglielmi G, Bryant D A, Stevens S E
出版信息
J Bacteriol. 1987 May;169(5):1830-5. doi: 10.1128/jb.169.5.1830-1835.1987.
In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa allophycocyanin subunits. Thus, these plastid genes are expressed in the cyanobacterium as polypeptides which become linked to a chromophore and are incorporated into the light-harvesting apparatus.
在蓝氏原绿藻（Cyanophora paradoxa）中，别藻蓝蛋白脱辅基蛋白亚基α和β由蓝小体（质体）基因组编码。这些基因通过质粒复制子转移到了蓝细菌聚球藻属（Synechococcus sp.）PCC 7002中。从转化后的蓝细菌中分离出的藻胆体被发现含有蓝氏原绿藻别藻蓝蛋白亚基。因此，这些质体基因在蓝细菌中作为多肽表达，这些多肽与发色团结合并被整合到光捕获装置中。
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