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藻蓝蛋白α和β亚基的基因。

Genes for the alpha and beta subunits of phycocyanin.

作者信息

de Lorimier R, Bryant D A, Porter R D, Liu W Y, Jay E, Stevens S E

出版信息

Proc Natl Acad Sci U S A. 1984 Dec;81(24):7946-50. doi: 10.1073/pnas.81.24.7946.

DOI:10.1073/pnas.81.24.7946
PMID:6096868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392270/
Abstract

Phycocyanin (PC) is a light-harvesting protein common to blue-green and red algae. We have isolated the genes for the two apoprotein subunits, alpha and beta, of PC from the blue-green alga Agmenellum quadruplicatum PR-6. We synthesized eight sense-strand tetradecameric oligonucleotide probes that could encode a particular pentapeptide in PC alpha from A. quadruplicatum. Only one probe hybridized with total RNA from this organism. This oligonucleotide showed homology to a unique restriction fragment when used to probe Southern blots of A. quadruplicatum DNA. The probe-homologous 3.1-kilobase pair HindIII fragment was cloned. The nucleotide sequence of a 1.7-kilobase pair segment of this clone was determined. Two open reading frames are contained in this region, which correspond in deduced amino acid sequence to PC alpha and PC beta subunits. Both coding sequences are in the same orientation, separated by 105 base pairs, with the PC beta gene 5' to the PC alpha gene. Each gene has a Shine-Dalgarno-type sequence near the initiation codon. Codon frequencies in the two genes may be correlated with the abundance of their products. The deduced amino acid sequences of the gene products show considerable homology with the alpha and beta PC subunits from other species.

摘要

藻蓝蛋白（PC）是蓝藻和红藻中常见的一种捕光蛋白。我们从蓝藻四聚顶丝藻PR-6中分离出了PC的两种脱辅基蛋白亚基α和β的基因。我们合成了8种有义链十四聚体寡核苷酸探针，这些探针可以编码四聚顶丝藻PCα中的特定五肽。只有一种探针与该生物体的总RNA杂交。当用于探测四聚顶丝藻DNA的Southern印迹时，该寡核苷酸与一个独特的限制性片段显示出同源性。克隆了与探针同源的3.1千碱基对的HindIII片段。测定了该克隆1.7千碱基对片段的核苷酸序列。该区域包含两个开放阅读框，其推导的氨基酸序列分别对应于PCα和PCβ亚基。两个编码序列方向相同，相隔105个碱基对，PCβ基因位于PCα基因的5'端。每个基因在起始密码子附近都有一个Shine-Dalgarno型序列。两个基因中的密码子频率可能与其产物的丰度相关。基因产物推导的氨基酸序列与其他物种的PCα和PCβ亚基显示出相当高的同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4823/392270/59f83dea1470/pnas00625-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4823/392270/59f83dea1470/pnas00625-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4823/392270/59f83dea1470/pnas00625-0286-a.jpg

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本文引用的文献

1
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Plant Physiol. 1983 Feb;71(2):409-19. doi: 10.1104/pp.71.2.409.
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Nitrogen Starvation and the Regulation of Glutamine Synthetase in Agmenellum quadruplicatum.氮饥饿与四重藻谷氨酰胺合成酶的调控。
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Transformation in Agmenellum quadruplicatum.Agmenellum quadruplicatum 的转化。
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Characterization of phycobiliprotein and linker polypeptide genes in Fremyella diplosiphon and their regulated expression during complementary chromatic adaptation.藻胆体蛋白和连接多肽基因在双鞭藻中的特性及其在互补色适应过程中的调控表达。
Photosynth Res. 1988 Jul;17(1-2):23-56. doi: 10.1007/BF00047680.
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Photoregulation of gene expression in the filamentous cyanobacterium Calothrix sp. PCC 7601: light-harvesting complexes and cell differentiation.丝状蓝藻 Calothrix sp. PCC 7601 中基因表达的光调控：捕光复合物和细胞分化。
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Nucleotide sequence and expression of the two genes encoding D2 protein and the single gene encoding the CP43 protein of Photosystem II in the cyanobacterium synechococcus sp. PCC 7002.蓝藻集胞藻 PCC 7002 中编码 D2 蛋白的两个基因和编码 PSII CP43 蛋白的单一基因的核苷酸序列及其表达。
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J Biol Chem. 1981 Dec 10;256(23):12167-75.
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Primary structure of phycocyanin from the unicellular rhodophyte Cyanidium caldarium. II. Complete amino acid sequence of the beta subunit.单细胞红藻嗜热栖热菌藻蓝蛋白的一级结构。II. β亚基的完整氨基酸序列。
J Biol Chem. 1981 Dec 10;256(23):12176-84.
6
Primary structure of allophycocyanin from the unicellular rhodophyte, Cyanidium caldarium. The complete amino acid sequences of the alpha and beta subunits.单细胞红藻嗜热栖热放线菌别藻蓝蛋白的一级结构。α和β亚基的完整氨基酸序列。
J Biol Chem. 1983 Aug 25;258(16):9931-40.
7
Phycobiliprotein synthesis in the unicellular rhodophyte, Cyanidium caldarium. Cell-free translation of the mRNAs for the alpha and beta subunit polypeptides of phycocyanin.单细胞红藻嗜热栖热菌中的藻胆蛋白合成。藻蓝蛋白α和β亚基多肽mRNA的无细胞翻译。
J Biol Chem. 1983 Apr 10;258(7):4503-10.
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Annu Rev Microbiol. 1982;36:173-98. doi: 10.1146/annurev.mi.36.100182.001133.
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J Biol Chem. 1982 Apr 10;257(7):3429-33.
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Hoppe Seylers Z Physiol Chem. 1981 Jun;362(6):611-28. doi: 10.1515/bchm2.1981.362.1.611.