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蓝藻藻胆体末端能量受体别藻蓝蛋白B编码基因的分离与分子特征分析

Isolation and molecular characterization of the gene encoding allophycocyanin B, a terminal energy acceptor in cyanobacterial phycobilisomes.

作者信息

Houmard J, Capuano V, Coursin T, Tandean de Marsac N

机构信息

C.N.R.S,UA1129, Départment de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France.

出版信息

Mol Microbiol. 1988 Jan;2(1):101-7.

PMID:3130540
Abstract

Phycobilisomes are the major constituents of the light-harvesting apparatus in both cyanobacteria and red algae and consist of a central core with radiating rods. From a genomic library of the cyanobacterium Calothrix 7601, a DNA fragment encoding allophycocyanin B, one of the two terminal energy acceptors of the core, was isolated and its nucleotide sequence was determined. Unlike all the other known genes encoding phycobiliproteins, the allophycocyanin B gene, apcD, is transcribed as a monocistronic unit. Mapping of the transcripts was performed and, in contrast to some of the Calothrix genes that encode rod components, transcription was shown to occur regardless of chromatic light received during cell growth.

摘要

藻胆体是蓝细菌和红藻中光捕获装置的主要组成部分,由一个带有辐射杆的中央核心组成。从蓝细菌7601的基因组文库中,分离出一个编码核心两个末端能量受体之一别藻蓝蛋白B的DNA片段,并测定了其核苷酸序列。与所有其他已知的编码藻胆蛋白的基因不同,别藻蓝蛋白B基因apcD作为一个单顺反子单元转录。对转录本进行了定位,与一些编码杆状成分的7601基因不同,结果表明,无论细胞生长过程中接收到何种有色光,转录都会发生。

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Mol Microbiol. 1988 Jan;2(1):101-7.
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Isolation and molecular characterization of the gene encoding allophycocyanin B, a terminal energy acceptor in cyanobacterial phycobillsomes.
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Photosynth Res. 1988 Oct;18(1-2):99-132. doi: 10.1007/BF00042981.
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Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803.
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Plant Cell. 2003 Oct;15(10):2448-63. doi: 10.1105/tpc.015016. Epub 2003 Sep 24.
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