Pashapoor A, Mashhadyrafie S, Mortazavi P
Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran, Islamic Republic Of.
Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran, Islamic Republic Of.
Folia Morphol (Warsz). 2020;79(1):113-119. doi: 10.5603/FM.a2019.0052. Epub 2019 May 7.
Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. The aim of this study was to assess oxidative stress and histological changes in the pancreas of alloxan-induced diabetic rats following Myristica fragrans seed (nutmeg) extract treatment.
Forty-eight male Wistar rats weighing 200-250 g were randomly divided into six groups of 8 rats each - group I, non-diabetic rats; group II, diabetic rats; groups III, IV and V, diabetic rats given orally nutmeg extract at levels of 50, 100 and 200 mg/kg, respectively; and group VI, diabetic rats given orally metformin (100 mg/kg). The experiment lasted for 28 days.
Data showed that nutmeg extract (100 and 200 mg/kg) significantly decreased the blood glucose levels and increased the levels of serum insulin in diabetic rats. Administration of nutmeg extract to diabetic rats reduced oxidative stress and improved the antioxidant activities in pancreatic tissue. Histopathologic results of treated groups revealed marked improvement in the morphology of the pancreas compared with the control diabetic group. In addition, number of pancreatic islets and per cent of β-cells increased significantly in these groups in comparison with diabetic untreated group.
These results suggest that nutmeg extract has potent antidiabetic and β-cell protection activities in alloxan induced diabetic rats, possibly via its antioxidant properties.
在替代医学体系中，许多传统疗法被推荐用于治疗糖尿病。本研究的目的是评估肉豆蔻种子提取物治疗后，四氧嘧啶诱导的糖尿病大鼠胰腺中的氧化应激和组织学变化。
将48只体重200 - 250克的雄性Wistar大鼠随机分为6组，每组8只——第一组，非糖尿病大鼠；第二组，糖尿病大鼠；第三组、第四组和第五组，分别给予50、100和200毫克/千克肉豆蔻提取物的糖尿病大鼠；第六组，给予口服二甲双胍（100毫克/千克）的糖尿病大鼠。实验持续28天。
数据显示，肉豆蔻提取物（100和200毫克/千克）显著降低糖尿病大鼠的血糖水平，并提高血清胰岛素水平。给糖尿病大鼠服用肉豆蔻提取物可降低氧化应激，并改善胰腺组织中的抗氧化活性。与糖尿病对照组相比，治疗组的组织病理学结果显示胰腺形态有明显改善。此外，与未治疗的糖尿病组相比，这些组中的胰岛数量和β细胞百分比显著增加。
这些结果表明，肉豆蔻提取物在四氧嘧啶诱导的糖尿病大鼠中具有有效的抗糖尿病和β细胞保护活性，可能是通过其抗氧化特性实现的。
