Cleveland J L, Rapp U R, Farrar W L
J Immunol. 1987 May 15;138(10):3495-504.
The cloned murine cytotoxic T cell line CT6 solely requires interleukin 2 (IL 2) for viability and cell cycle progression. Treatment of G arrested cultures of CT6 cells with recombinant IL 2 induces the rapid sequential expression of the nuclear proto-oncogenes c-fos, c-myc, and c-myb but does not affect the expression of several cytosolic or membrane-associated proto-oncogenes. A comparison of early genes induced by growth factor treatment of quiescent NIH/3T3 fibroblasts and CT6 cells demonstrated that only c-fos and c-myc induction is shared in the two different lineages. Factor-independent lines derived from CT6 cells show no mitogenic response to IL 2, yet binding of IL 2 with its receptor in the cells was capable of inducing the expression of c-fos and c-myc. In factor-independent cell lines, c-myc was uniformly expressed at high constitutive levels, suggesting that c-myc abrogates growth factor requirements of these cells. The levels of c-myc expression in the factor-independent lines was not due to an autocrine production of IL 2 but may be a consequence of constitutively activated IL 2 receptors.
克隆的小鼠细胞毒性T细胞系CT6的存活和细胞周期进展仅依赖白细胞介素2（IL-2）。用重组IL-2处理处于G期停滞的CT6细胞培养物，可诱导核原癌基因c-fos、c-myc和c-myb快速顺序表达，但不影响几种胞质或膜相关原癌基因的表达。比较生长因子处理静止的NIH/3T3成纤维细胞和CT6细胞所诱导的早期基因，发现只有c-fos和c-myc的诱导在这两种不同细胞系中是共同的。源自CT6细胞的不依赖因子的细胞系对IL-2无促有丝分裂反应，但IL-2与其在细胞中的受体结合能够诱导c-fos和c-myc的表达。在不依赖因子的细胞系中，c-myc以较高的组成水平均匀表达，这表明c-myc消除了这些细胞对生长因子的需求。不依赖因子的细胞系中c-myc的表达水平并非由于IL-2的自分泌产生，而可能是组成性激活的IL-2受体的结果。
