Reed J C, Alpers J D, Scherle P A, Hoover R G, Nowell P C, Prystowsky M B
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.
Oncogene. 1987 May;1(2):223-8.
We have compared the effects of a mitogenic lectin, concanavalin A (Con A), and a growth factor, interleukin-2 (IL-2), on the expression of the c-myc, c-fos, and c-myb proto-oncogenes in cloned T lymphocytes. Accumulation of c-myc mRNA was induced by both ConA and IL-2 in these cells. In contrast, expression of c-fos was stimulated primarily by ConA, and accumulation of c-myb mRNA was induced predominantly by IL-2. Thus, ConA and IL-2 induce expression of overlapping, but non-identical, sets of proto-oncogenes in T lymphocytes. Investigations with several different cloned T cells revealed that: (1) c-myb is not induced in all T cells stimulated to grow, indicating that its expression may not be absolutely required for their proliferation; and (2) expression of c-myc, even in combination with c-fos, can be insufficient for growth, demonstrating functional differences between cellular and viral oncogenes in T cells. These observations provide insight into the roles of the c-myc, c-fos, and c-myb proto-oncogenes in normal T cell responses.
我们比较了促有丝分裂凝集素刀豆球蛋白A（Con A）和生长因子白细胞介素-2（IL-2）对克隆T淋巴细胞中c-myc、c-fos和c-myb原癌基因表达的影响。在这些细胞中，Con A和IL-2均诱导c-myc mRNA的积累。相比之下，c-fos的表达主要由Con A刺激，而c-myb mRNA的积累主要由IL-2诱导。因此，Con A和IL-2在T淋巴细胞中诱导重叠但不相同的原癌基因表达。对几种不同克隆T细胞的研究表明：（1）并非所有被刺激生长的T细胞中c-myb都会被诱导，这表明其表达可能并非T细胞增殖绝对必需的；（2）即使c-myc与c-fos共同表达，也可能不足以促进生长，这证明了细胞癌基因和病毒癌基因在T细胞中的功能差异。这些观察结果为c-myc、c-fos和c-myb原癌基因在正常T细胞反应中的作用提供了深入了解。
