Kaplan G, Gandhi R R, Weinstein D E, Levis W R, Patarroyo M E, Brennan P J, Cohn Z A
J Immunol. 1987 May 1;138(9):3028-34.
The extent to which M. leprae and its products induced suppression of T lymphocyte proliferation in vitro was evaluated. M. leprae antigens suppressed T cell proliferation in response to mitogens and antigens in both lepromatous and tuberculoid patients, as well as controls never exposed to M. leprae or M. leprae endemic areas. Both soluble and particulate fractions of M. leprae were found to suppress proliferation in a dose-dependent manner. The extent of suppression was inversely related to the proliferative response of the donors mononuclear cells to M. leprae. Evidence indicates that M. leprae contains both stimulatory and suppressive molecules for T cells. One such suppressive antigen, Lipoarabinomannan (LAM)-B of M. leprae, also suppressed the proliferative response of tuberculoid patients. Suppression was also observed with the LAM-B of M. tuberculosis. The suppressive effects observed were not due to the toxicity of the antigen. Some of the suppressive activity was mediated by T8+ suppressor cells and was expressed in both lepromatous and tuberculoid patients. We suggest that previous sensitization to M. leprae and other cross-reactive mycobacterial antigens determines the sensitivity of T cells to the suppressive effects of M. leprae antigens.
评估了麻风分枝杆菌及其产物在体外诱导T淋巴细胞增殖抑制的程度。麻风分枝杆菌抗原抑制了瘤型和结核样型患者以及从未接触过麻风分枝杆菌或麻风分枝杆菌流行地区的对照者对丝裂原和抗原的T细胞增殖。发现麻风分枝杆菌的可溶性和颗粒性部分均以剂量依赖性方式抑制增殖。抑制程度与供体单核细胞对麻风分枝杆菌的增殖反应呈负相关。有证据表明，麻风分枝杆菌含有对T细胞具有刺激和抑制作用的分子。一种这样的抑制性抗原，即麻风分枝杆菌的脂阿拉伯甘露聚糖（LAM）-B，也抑制了结核样型患者的增殖反应。结核分枝杆菌的LAM-B也观察到了抑制作用。观察到的抑制作用并非由于抗原的毒性。一些抑制活性由T8 +抑制细胞介导，在瘤型和结核样型患者中均有表达。我们认为，先前对麻风分枝杆菌和其他交叉反应性分枝杆菌抗原的致敏决定了T细胞对麻风分枝杆菌抗原抑制作用的敏感性。
