Zhou Yongzhuang, Zickus Vytautas, Zammit Paul, Taylor Jonathan M, Harvey Andrew R
School of Physics & Astronomy, University of Glasgow, Glasgow, G12 8QQ, UK.
Biomed Opt Express. 2018 Nov 26;9(12):6444-6454. doi: 10.1364/BOE.9.006444. eCollection 2018 Dec 1.
Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there has been no technique reported for snapshot imaging of thick samples in large volumes with high precision. We have combined computational microscopy and the diffraction-free, self-bending property of Airy-beams to track fluorescent beads with sub-micron precision through an extended axial range (up to 600 μm) within the flowing blood of 3 days post-fertilization (dpf) zebrafish embryos. The spatial trajectories of the tracer beads within flowing blood were recorded during transit through both cardinal and intersegmental vessels, and the trajectories were found to be consistent with the segmentation of the vasculature recorded using selective-plane illumination microscopy (SPIM). This method provides sufficiently precise spatial and temporal measurement of 3D blood flow that has the potential for directly probing key biomechanical quantities such as wall shear stress, as well as exploring the fluidic repercussions of cardiovascular diseases. Although we demonstrate the technique for blood flow, the ten-fold better enhancement in the depth range offers improvements in a wide range of applications of high-speed precision measurement of fluid flow, from microfluidics through measurement of cell dynamics to macroscopic aerosol characterizations.
对活生物体中的血流进行实验表征对于理解心血管系统的发育和功能至关重要，但尚未有技术能够高精度地对大体积厚样本进行快照成像。我们将计算显微镜与艾里光束的无衍射、自弯曲特性相结合，在受精后3天（dpf）斑马鱼胚胎的流动血液中，通过扩展的轴向范围（高达600μm）以亚微米精度追踪荧光珠。示踪珠在流动血液中通过主血管和节间血管时的空间轨迹被记录下来，并且发现这些轨迹与使用选择性平面照明显微镜（SPIM）记录的血管系统分割结果一致。该方法提供了对三维血流足够精确的空间和时间测量，有可能直接探测关键的生物力学量，如壁面剪应力，以及探索心血管疾病的流体动力学影响。尽管我们展示了该血流技术，但深度范围提高了十倍，在从微流体到细胞动力学测量再到宏观气溶胶表征的广泛流体流动高速精密测量应用中都有改进。
