Institut für Mikrobiologie und Biotechnologie, Universität Ulm , Albert-Einstein-Allee 11 , 89081 Ulm , Germany.
Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland (HES-SO Valais) , Route du Rawyl 64 , 1950 Sion , Switzerland.
Biomacromolecules. 2019 Sep 9;20(9):3271-3282. doi: 10.1021/acs.biomac.9b00342. Epub 2019 May 15.
Anaerobic production of the biopolymer poly(3-hydroxybutyrate) (PHB) and the monomer 3-hydroxybutyrate (3-HB) was achieved using recombinant clostridial acetogens supplied with syn(thesis) gas as the sole carbon and energy source. 3-HB production was successfully accomplished by a new synthetic pathway containing the genes (encoding thiolase A), (encoding CoA-transferase A/B), and (encoding ()-3-hydroxybutyrate dehydrogenase). The respective recombinant [p83_tcb] strain produced autotrophically 0.98 ± 0.12 mM and heterotrophically 21.7 ± 0.27 mM 3-HB. As a proof of concept, production of PHB was achieved using recombinant and strains expressing a novel synthetic PHB pathway containing the genes (encoding thiolase A), (encoding 3-hydroxybutyryl-CoA dehydrogenase), (encoding crotonase), (encoding ()-enoyl-CoA hydratase), and (encoding PHA synthase). The strain [p83_PHB_Scaceti] synthesized heterotrophically 3.4 ± 0.29% PHB per cell dry weight (CDW) and autotrophically 1.12 ± 0.12% PHB per CDW.
利用重组梭菌乙酰辅酶 A 合成菌作为唯一的碳源和能源，实现了生物聚合物聚（3-羟基丁酸酯）（PHB）和单体 3-羟基丁酸（3-HB）的厌氧生产。通过包含基因（编码硫解酶 A）、（编码 CoA-转移酶 A/B）和（编码（）-3-羟基丁酸脱氢酶）的新合成途径成功实现了 3-HB 的生产。相应的重组 [p83_tcb] 菌株以自养方式产生 0.98 ± 0.12 mM 和异养方式产生 21.7 ± 0.27 mM 3-HB。作为概念验证，使用表达包含基因（编码硫解酶 A）、（编码 3-羟基丁酰-CoA 脱氢酶）、（编码巴豆酰辅酶 A 脱羧酶）、（编码（）-烯酰-CoA 水合酶）和（编码 PHA 合酶）的新型合成 PHB 途径的重组和菌株实现了 PHB 的生产。菌株 [p83_PHB_Scaceti] 以异养方式合成 3.4 ± 0.29% PHB 每细胞干重（CDW），以自养方式合成 1.12 ± 0.12% PHB 每 CDW。
