Department of chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Environ Toxicol. 2019 Aug;34(8):941-949. doi: 10.1002/tox.22765. Epub 2019 May 8.
This research was conducted to investigate the biochemical effects of thymosin alpha-1 using human lung cancer cells (A549). The A549 cells were treated with different concentrations of Thα1 for 24 h and the growth, inhibition of cells was determined. Thα1 revealed anti-proliferative effect at 24 and 48 μg/ml after 24 h. Furthermore, it indicated antioxidant properties by significantly enhancing the activity of catalase (12 μg/ml), superoxide dismutase (6 and 12 μg/ml), and glutathione peroxidase (3, 6 and 12 μg/ml) and reducing the production of cellular ROS. Our results showed that Thα1 inhibits the migration of A549 cells in a concentration-dependent manner after 24 and 48 h. Moreover, the effect of Thα1 on apoptosis was investigated by Hoechst 33342 staining and cell cycle analysis. Results demonstrated no significant effect on the induction of apoptosis in A549 cells. In conclusion, our results showed the antioxidant properties of Thα1 on A549 cancer cells.
本研究旨在探讨胸腺肽 α-1（Thα1）对人肺癌细胞（A549）的生化作用。将 A549 细胞用不同浓度的 Thα1 处理 24 小时，测定细胞的生长、抑制情况。24 小时后，Thα1 在 24 和 48 μg/ml 时表现出抗增殖作用。此外，它通过显著增强过氧化氢酶（12 μg/ml）、超氧化物歧化酶（6 和 12 μg/ml）和谷胱甘肽过氧化物酶（3、6 和 12 μg/ml）的活性，并降低细胞 ROS 的产生，显示出抗氧化特性。我们的结果表明，Thα1 在 24 和 48 小时后以浓度依赖的方式抑制 A549 细胞的迁移。此外，通过 Hoechst 33342 染色和细胞周期分析研究了 Thα1 对细胞凋亡的影响。结果表明，Thα1 对 A549 细胞凋亡的诱导没有显著影响。总之，我们的结果表明 Thα1 对 A549 癌细胞具有抗氧化特性。
