Faraoni Paola, Bellumori Maria, Cecchi Lorenzo, Zonfrillo Beatrice, Innocenti Marzia, Gnerucci Alessio, Mulinacci Nadia, Ranaldi Francesco
Department of Experimental and Clinic Biomedical Sciences "Mario Serio", University of Florence, Viale Pieraccini 6, 50139 Florence, FI, Italy.
Department of NEUROFARBA, Division of Pharmaceutical and Nutraceutical Sciences, University of Florence, Via U. Schiff 6, 50019 Sesto Fiorentino, FI, Italy.
Antioxidants (Basel). 2023 Jun 27;12(7):1347. doi: 10.3390/antiox12071347.
The effects of the phenolic compounds of extra virgin olive oil (EVOO) on AGS cells have never been studied so far, which is the aim of this study. The profiles of the main phenolic components in EVOOs, mainly secoiridoid compounds derived from the transformation of oleuropein during the olive milling process, were evaluated and compared. Oils of different origins were evaluated aiming at verifying whether chemical differences in the phenolic composition of the dry extracts played a role in the metabolism and in maintaining the cellular redox state of AGS cells. The following key enzymes of some metabolic pathways were studied: lactate dehydrogenase, enolase, pyruvate kinase, glucose 6-phosphate dehydrogenase, citrate synthase, 3-Hydroxyacyl-CoA dehydrogenase and hexokinase. As confirmed through PCA analysis, pretreatments with the dry extracts of EVOOs at different concentrations appeared to be able to counteract the enzymatic activity alterations due to oxidative stress induced by HO 1 mM and 2 mM. The studied phytocomplexes showed the ability to protect AGS cells from oxidative damage and the secoiridoid derivatives from both oleuropein and ligstroside contributed to the observed effects. The results suggested that EVOOs with medium to high concentrations of phenols can exert this protection.
迄今为止，从未研究过特级初榨橄榄油（EVOO）中的酚类化合物对AGS细胞的影响，而这正是本研究的目的。对EVOO中主要酚类成分的概况进行了评估和比较，这些成分主要是橄榄研磨过程中橄榄苦苷转化产生的裂环烯醚萜类化合物。对不同产地的橄榄油进行评估，旨在验证干提取物中酚类成分的化学差异是否在AGS细胞的代谢和维持细胞氧化还原状态中发挥作用。研究了一些代谢途径的以下关键酶：乳酸脱氢酶、烯醇化酶、丙酮酸激酶、葡萄糖6-磷酸脱氢酶、柠檬酸合酶、3-羟基酰基辅酶A脱氢酶和己糖激酶。正如通过主成分分析（PCA）所证实的那样，用不同浓度的EVOO干提取物进行预处理似乎能够抵消由1 mM和2 mM HO诱导的氧化应激所引起的酶活性改变。所研究的植物复合物显示出保护AGS细胞免受氧化损伤的能力，来自橄榄苦苷和裂环橄榄苦苷的裂环烯醚萜衍生物促成了所观察到的效果。结果表明，酚类含量中等至高的EVOO能够发挥这种保护作用。
Zotero 插件
