Jezequel A M, Macarri G, Rinaldesi M L, Venturini C, Lorenzini I, Orlandi F
Liver. 1986 Dec;6(6):341-9. doi: 10.1111/j.1600-0676.1986.tb00302.x.
Horseradish peroxidase (HRP) and lanthanum chloride (LaCl3) are useful tools for, respectively, the study of vesicular transport through the hepatocyte and the study of the permeability of junctional complexes. These tracers have been used to detect the changes associated with the choleresis independent of bile acids induced by valproic acid (VPA) in rats. The animals were given a single dose of VPA (600 mg/kg, ip). HRP (100 mg/kg) or 5 mM LaCl3 were given intraportally after 1 h, when bile flow had increased twofold. The excretion of HRP in bile was measured colorimetrically up to 2 h after HRP. Ultrastructural morphometry was conducted on liver of intact rats taken from 1 to 40 min after HRP. The volume density (VD) of HRP-containing vesicles and of HRP-containing multivesicular bodies (MVB) was counted. In VPA-treated rats, HRP appeared in bile with a peak showing at 5 min against 20 min in controls, but the total amount of HRP excreted was less than in controls. The intrahepatocytic vesicular transport of HRP was also modified, showing a peak at 3 min in VPA-treated rats compared to 10 min in controls, together with a decreased VD of pericanalicular vesicles. This was accompanied by an increase of HRP-containing MVB, already evident at 5 min.(ABSTRACT TRUNCATED AT 250 WORDS)
辣根过氧化物酶（HRP）和氯化镧（LaCl3）分别是研究肝细胞囊泡运输和连接复合体通透性的有用工具。这些示踪剂已被用于检测与大鼠丙戊酸（VPA）诱导的胆汁酸无关的胆汁分泌相关变化。给动物单次腹腔注射VPA（600 mg/kg）。1小时后，当胆汁流量增加两倍时，经门静脉给予HRP（100 mg/kg）或5 mM LaCl3。在给予HRP后2小时内用比色法测量胆汁中HRP的排泄量。对HRP注射后1至40分钟处死的完整大鼠肝脏进行超微结构形态计量学分析。计算含HRP囊泡和含HRP多囊泡体（MVB）的体积密度（VD）。在VPA处理的大鼠中，HRP在胆汁中出现的峰值出现在5分钟，而对照组为20分钟，但排泄的HRP总量低于对照组。HRP在肝内的囊泡运输也发生了改变，VPA处理的大鼠在3分钟出现峰值，而对照组为10分钟，同时胆小管周围囊泡的VD降低。这伴随着含HRP的MVB增加，在5分钟时就已明显。（摘要截断于250字）
