Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Int J Biol Macromol. 2019 Aug 1;134:91-99. doi: 10.1016/j.ijbiomac.2019.04.195. Epub 2019 May 5.
The chitosan-based hydrogel dressings have been intensively engaged in wound healing. In respect to the relatively unsatisfied anti-bacterial performance of chitosan, we developed a self-healable cordycepin (abbreviated to CY)/chitosan (abbreviated to CS) hydrogel dressing cross linking by non-covalent bonds (hydrogen bond and electrostatic interaction) for wound healing through one-step 'freeze-thaw' method without adding any cross-linking agent. The as-prepared hybrid hydrogel exhibited excellent biocompatibility, suitable swelling ratio and desired mechanical strength, providing remarkable antimicrobial effect with no side effects in vitro. The self-healable property enables this hydrogel to adapt irregularly shaped wound defects without premolding. Moreover, our in vivo experiments confirmed that CY/CS hydrogel served as a quicker re-epithelization of skin wounds and an apparent increasing collagen deposition compared with chitosan hydrogels. Additionally, the CY/CS hydrogel significantly increased the expressions of epithelial regeneration markers including laminin and involucrin. The present study highlights a facile hydrogel strategy to balance and optimize the biocompatibility, swelling ratio and self-adapting ability for treating wound healing.
壳聚糖基水凝胶敷料在伤口愈合方面得到了广泛的关注。鉴于壳聚糖相对不理想的抗菌性能，我们开发了一种通过非共价键（氢键和静电相互作用）交联的自修复虫草素（简称 CY）/壳聚糖（简称 CS）水凝胶敷料，通过一步“冻融”法制备，无需添加任何交联剂。所制备的杂化水凝胶表现出优异的生物相容性、合适的溶胀比和理想的机械强度，在体外具有显著的抗菌效果，无副作用。自修复性能使这种水凝胶能够适应不规则形状的伤口缺陷，无需预成型。此外，我们的体内实验证实，与壳聚糖水凝胶相比，CY/CS 水凝胶可加速皮肤伤口的再上皮化，并明显增加胶原蛋白的沉积。此外，CY/CS 水凝胶显著增加了上皮再生标志物包括层粘连蛋白和内披蛋白的表达。本研究强调了一种简单的水凝胶策略，以平衡和优化生物相容性、溶胀比和自适应能力，用于治疗伤口愈合。
