Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, 832003, Xinjiang, China.
Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, 832003, Xinjiang, China.
Sci Rep. 2019 May 8;9(1):7092. doi: 10.1038/s41598-019-43582-z.
Using paraffin sections, the stages of walnut female flower bud differentiation were divided into the predifferentiation period (F_1), initial differentiation period (F_2) and flower primordium differentiation period (F_3). Leaf buds collected at the same stage as F_2 were designated JRL. Transcriptomic profiling was performed, and a total of 132,154 unigenes were obtained with lengths ranging from 201 bp to 16,831 bp. The analysis of differentially expressed genes (DEGs) showed that there were 597, 784 and 532 DEGs in the three combinations F_1vsF_2, F_1vsF_3, and F_2vsF_3, respectively. The comparison F_2vsJRL showed that 374 DEGs were differentially expressed between female buds and leaf buds. Thirty-one DEGs related to flowering time were further used to construct coexpression networks, and CRY2 and NF-YA were identified as core DEGs in flowering time regulation. Eighteen DEGs related to flowering time were subjected to real-time quantitative analysis. Our work provides a foundation for further research on the walnut floral transition and provides new resources for future research on walnut biology and biotechnology.
利用石蜡切片，将核桃雌花芽分化的阶段分为分化前（F_1）、初始分化期（F_2）和花原基分化期（F_3）。与 F_2 同期采集的叶芽被指定为 JRL。进行了转录组谱分析，共获得长度为 201bp 至 16831bp 的 132154 个基因。差异表达基因（DEGs）分析表明，在 F_1vsF_2、F_1vsF_3 和 F_2vsF_3 的三个组合中，分别有 597、784 和 532 个 DEGs。F_2vsJRL 的比较表明，雌花芽和叶芽之间有 374 个 DEGs 差异表达。进一步将 31 个与开花时间相关的 DEGs 用于构建共表达网络，鉴定出 CRY2 和 NF-YA 是开花时间调控的核心 DEGs。对 18 个与开花时间相关的 DEGs 进行了实时定量分析。我们的工作为核桃花转变的进一步研究提供了基础，并为未来核桃生物学和生物技术的研究提供了新的资源。
