深入探讨参与. 地下花芽分化的主要代谢物

Insights into the Major Metabolites Involved in the Underground Floral Differentiation of .

机构信息

School of Life Sciences, Tonghua Teachers College, Tonghua, China.

College of Agriculture, Jilin Agricultural University, Jilin, China.

出版信息

Biomed Res Int. 2022 May 13;2022:7431151. doi: 10.1155/2022/7431151. eCollection 2022.

DOI:10.1155/2022/7431151

PMID:35601148

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9122723/

Abstract

BACKGROUND

Decne (Liliaceae) is an early spring ephemeral with an underground initial floral differentiation stage. The flowering mechanism is crucial in ornamental plants due to the associated economic value. Therefore, this study is aimed at exploring the metabolic landscape during floral differentiation, including flower primordium, perianth, stamen, and the pistil differentiation period, in coupled with a conjoint analysis of the metabolome and transcriptome. Using ultraperformance liquid chromatography-tandem mass spectrometry, we identified 586 metabolites from 13 major metabolite classes. Comparative metabolomics between different floral developmental stages revealed several abundant metabolites during the respective phases. Upaccumulation of p-coumaroylputrescine, scopoletin, isorhoifolin, cosmosiin, genistin, and LysoPC 15 : 0 emphasized the significance of these compounds during flower development. Furthermore, previously identified DEGs, viz., , , , and for floral differentiation, depicted a high correlation with lipid, flavonoid, and phenolics accumulation during floral developmental stages.

CONCLUSIONS

Together, the results improve our interpretation of the underground floral development in .

摘要

背景

Decne（百合科）是一种早春的短命植物，具有地下初始花分化阶段。由于与相关的经济价值有关，开花机制在观赏植物中至关重要。因此，本研究旨在探索花分化过程中的代谢景观，包括花原基、花被、雄蕊和雌蕊分化期，并结合代谢组学和转录组学的联合分析。使用超高效液相色谱-串联质谱法，我们从 13 个主要代谢物类别中鉴定出 586 种代谢物。不同花发育阶段之间的比较代谢组学揭示了各个阶段的几种丰富代谢物。在各自的阶段中，p-对香豆酰腐胺、山柰酚、异瑞福林、cosmosiin、金雀异黄素和 LysoPC 15:0 的积累增加强调了这些化合物在花发育过程中的重要性。此外，先前鉴定的用于花分化的差异表达基因，即、、、和，与花发育阶段的脂质、类黄酮和酚类物质积累高度相关。