Stern G A, Zam Z S
Ophthalmology. 1987 Feb;94(2):115-9. doi: 10.1016/s0161-6420(87)33492-x.
Previous studies have demonstrated that Pseudomonas aeruginosa adheres more readily to soft contact lenses with a mucin coating than to unworn contact lenses. The mucin coatings that develop on soft contact lenses may, therefore, play a significant role in the pathogenesis of contact lens-associated Pseudomonas corneal ulceration. We tested the ability of a variety of enzymatic contact lens cleaners and other enzyme solutions to decrease the adherence of Pseudomonas to mucin-coated soft contact lenses. Of the commercially available solutions that were tested, cleaning with Optizyme and Extenzyme significantly reduced the adherence of Pseudomonas to the lenses, whereas cleaning with the Softmate Weekly Cleaning System had no effect. Optizyme and Extenzyme were as effective as a 10% solution of acetylcysteine and more effective than a 0.25% trypsin solution. Neuraminidase at pH 5 was the most effective solution at reducing the adherence of Pseudomonas to the lenses, supporting the finding that sialic acid is a specific receptor for Pseudomonas aeruginosa. Soft contact lenses should be cleaned frequently with an effective enzymatic cleaner to reduce the likelihood of Pseudomonas adhering to the lens and thereby reduce the incidence of Pseudomonas corneal ulceration in soft contact lens wearers.
先前的研究表明，与未佩戴过的隐形眼镜相比，铜绿假单胞菌更容易附着在带有粘蛋白涂层的软性隐形眼镜上。因此，软性隐形眼镜上形成的粘蛋白涂层可能在与隐形眼镜相关的铜绿假单胞菌角膜溃疡发病机制中起重要作用。我们测试了多种酶促隐形眼镜清洁剂和其他酶溶液降低铜绿假单胞菌对粘蛋白涂层软性隐形眼镜附着力的能力。在所测试的市售溶液中，使用Optizyme和Extenzyme清洁可显著降低铜绿假单胞菌对镜片的附着力，而使用Softmate每周清洁系统清洁则没有效果。Optizyme和Extenzyme与10%的乙酰半胱氨酸溶液效果相当，且比0.25%的胰蛋白酶溶液更有效。pH值为5的神经氨酸酶是降低铜绿假单胞菌对镜片附着力最有效的溶液，这支持了唾液酸是铜绿假单胞菌特异性受体的发现。软性隐形眼镜应经常使用有效的酶促清洁剂进行清洁，以降低铜绿假单胞菌附着在镜片上的可能性，从而降低软性隐形眼镜佩戴者中铜绿假单胞菌角膜溃疡的发生率。
