Korber B, Hood L, Stroynowski I
Proc Natl Acad Sci U S A. 1987 May;84(10):3380-4. doi: 10.1073/pnas.84.10.3380.
Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2Ld and H-2Dd DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant alpha and gamma interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.
干扰素可调节大量哺乳动物基因的表达，包括主要组织相容性抗原基因。为了研究干扰素作用的机制，我们分析了小鼠H-2Ld和H-2Dd DNA序列控制对干扰素反应的能力。结果表明，I类基因表达的干扰素调节是复杂的，涉及至少两种机制，这两种机制依赖于转录起始位点上游和下游的I类序列。在转染的小鼠L细胞中，这两个区域对于I类基因表达的完全增强都是必需的，大部分反应由位于转录起始位点3'端的序列控制。对5'区域编码反应的精细定位分析还表明，重组α和γ干扰素可能通过使用不同的顺式作用调节序列对I类基因表达发挥作用。
