Chankvetadze Bezhan
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Methods Mol Biol. 2019;1985:93-126. doi: 10.1007/978-1-4939-9438-0_6.
This chapter summarizes the application of polysaccharide-based chiral stationary phases (CSPs) for separation of enantiomers in high-performance liquid chromatography (HPLC). Since this book contains dedicated chapters on enantioseparations using supercritical fluid chromatography (SFC), or capillary electrochromatography (CEC), the application of polysaccharide-based materials in these modes of liquid-phase separation techniques is touched just superficially. Special emphasis is directed toward a discussion of the optimization of polysaccharide-based chiral selectors, their attachment onto the carrier, and the optimization of the support. The optimization of the separation of enantiomers based on various parameters such as mobile phase composition and temperature is discussed.
本章总结了基于多糖的手性固定相（CSPs）在高效液相色谱（HPLC）中用于对映体分离的应用。由于本书包含关于使用超临界流体色谱（SFC）或毛细管电色谱（CEC）进行对映体分离的专门章节，因此仅简要提及了基于多糖的材料在这些液相分离技术模式中的应用。重点讨论了基于多糖的手性选择剂的优化、它们在载体上的附着以及载体的优化。还讨论了基于流动相组成和温度等各种参数对对映体分离的优化。
