用于高效液相色谱法通用对映体分离的功能化环糊精点击手性固定相

Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC.

作者信息

Zhou Jie, Tang Jian, Tang Weihua

机构信息

Key Laboratory of Soft Chemistry and Functional Materials, Ministry of Education, Nanjing University of Science and Technology, Nanjing, People's Republic of China.

出版信息

Methods Mol Biol. 2019;1985:147-157. doi: 10.1007/978-1-4939-9438-0_8.

DOI:10.1007/978-1-4939-9438-0_8

PMID:31069733

Abstract

The urgent demand for pure biological and pharmaceutical enantiomers has brought together great efforts in developing chiral techniques. High-performance liquid chromatography employing chiral stationary phases (CSPs) has evolved as a powerful tool for both chiral analysis and manufacture of pure enantiomers. Herein, we describe a facile method to prepare a phenylcarbamate cyclodextrin (CD)-based CSPs via azide/alkyne click chemistry. The functionalities of CD rims are altered to mediate the enantioseparation performance in multimode high-performance liquid chromatography.

摘要

对纯生物和药物对映体的迫切需求促使人们在开发手性技术方面付出了巨大努力。采用手性固定相（CSPs）的高效液相色谱已发展成为一种用于手性分析和制备纯对映体的强大工具。在此，我们描述了一种通过叠氮化物/炔烃点击化学制备基于苯基氨基甲酸酯环糊精（CD）的CSPs的简便方法。改变CD边缘的官能团以调节多模式高效液相色谱中的对映体分离性能。