Wang Yong, Ng Siu Choon
Department of Chemistry, School of Sciences, Tianjin University, Tianjin, China.
Methods Mol Biol. 2013;970:69-79. doi: 10.1007/978-1-62703-263-6_4.
Cyclodextrin-bonded silica material is one of the most commonly used chiral stationary phases in liquid chromatography for pharmaceutical analysis and enantioseparations. The approaches for immobilization of cyclodextrins onto the silica surface influence both, the stability of the chiral stationary phase and the enantioselectivity. In this chapter, we describe an established example of the preparation of a cyclodextrin-based chiral stationary phase via "click chemistry" and their application for enantioseparations of racemic compounds by HPLC.
环糊精键合硅胶材料是液相色谱中用于药物分析和对映体分离最常用的手性固定相之一。将环糊精固定在硅胶表面的方法会影响手性固定相的稳定性和对映体选择性。在本章中，我们描述了一个通过“点击化学”制备基于环糊精的手性固定相的既定示例，以及它们在通过高效液相色谱法拆分外消旋化合物中的应用。
