Castro-Puyana María, Marina María Luisa
Departamento de Química Analítica, Química Física e Ingeniería Química, Facultad de Ciencias, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Methods Mol Biol. 2019;1985:391-405. doi: 10.1007/978-1-4939-9438-0_23.
Capillary electrophoresis (CE) is a well-established and one of the most powerful separation techniques in the field of chiral separations. Its hyphenation with mass spectrometry (MS) combines both the high separation efficiency and low sample consumption of CE and the high sensitivity and structural information of MS. Thus, the outstanding chiral resolution power of CE along with the MS advantages makes CE-MS a perfect combination to achieve sensitive enantioseparations. This chapter describes three representative examples of different approaches used in the chiral analysis of amino acids in biological fluids by CE-MS. The first methodology uses the partial filling technique to avoid the entry of cyclodextrins in the MS source. The second method shows the possibility to carry out the direct coupling EKC-MS even when a relative high concentration of a native cyclodextrin is used as chiral selector. The last example illustrates an alternative strategy based on the formation of stable diastereomers between an enantiomerically pure chiral reagent and the amino acids enantiomers which can be separated in an achiral environment.
毛细管电泳（CE）是一种成熟且在手性分离领域最强大的分离技术之一。它与质谱（MS）联用，兼具了CE的高分离效率和低样品消耗以及MS的高灵敏度和结构信息。因此，CE出色的手性拆分能力与MS的优势相结合，使得CE-MS成为实现灵敏对映体分离的完美组合。本章介绍了通过CE-MS对手性生物流体中的氨基酸进行分析时所采用的三种不同方法的代表性示例。第一种方法使用部分填充技术以避免环糊精进入质谱源。第二种方法表明，即使使用相对高浓度的天然环糊精作为手性选择剂，也有可能进行直接耦合EKC-MS。最后一个示例说明了一种基于对映体纯手性试剂与氨基酸对映体之间形成稳定非对映异构体的替代策略，这些非对映异构体可以在非手性环境中分离。
