Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.
Molecules. 2022 Jun 27;27(13):4126. doi: 10.3390/molecules27134126.
It is now more than 25 years since the first report of enantioselective analysis by capillary electrophoresis-mass spectrometry (CE-MS) appeared. This article reviews the power of chiral CE-MS in resolving issues on the use of chiral selector incompatibility with MS and poor detectability encountered for chiral compounds by UV detection. The review begins with the general principles, requirements, and critical aspects of chiral CE-MS instrumentation. Next, the review provides a survey of MS-compatible chiral selectors (CSs) reported during the past decade, and the key achievements encountered in the time period using these CSs. Within the context of the strategies used to combine CE and MS, special attention is paid to the approaches that feature partial filling technique, counter-migration techniques, and direct use of CS, such as molecular micelles. In particular, the development and application of moving and fixed CS for EKC-MS, MEKC-MS, and CEC-MS demonstrate how various chiral compounds analyses were solved in a simple and elegant way during the 2010-2020 review period. The most noteworthy applications in the determination of chiral compounds are critically examined. The operating analytical conditions are detailed in the Tables, and the authors provide commentary on future trends of chiral separations by CE-MS.
自第一篇手性毛细管电泳-质谱（CE-MS）分析的报告发表以来，已经过去了 25 年以上。本文综述了手性 CE-MS 在解决手性选择剂与 MS 不兼容以及 UV 检测对手性化合物检测灵敏度差的问题方面的强大功能。综述首先介绍了手性 CE-MS 仪器的一般原理、要求和关键方面。接下来，综述了过去十年中报道的 MS 兼容手性选择剂 (CS)，并在手性 CS 应用期间取得了关键成就。在手性 CE 和 MS 结合的策略方面，特别关注部分填充技术、逆流技术以及 CS 的直接应用（如分子胶束）等方法。特别是，用于 EKC-MS、MEKC-MS 和 CEC-MS 的移动和固定 CS 的发展和应用，展示了在 2010-2020 年的综述期间，如何以简单优雅的方式解决各种手性化合物分析的问题。对手性化合物测定中最值得注意的应用进行了批判性检查。表中详细列出了操作分析条件，作者对手性 CE-MS 分离的未来趋势进行了评论。
