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驱动蛋白与海胆有丝分裂纺锤体的一种非微管成分相关联。

Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.

作者信息

Leslie R J, Hird R B, Wilson L, McIntosh J R, Scholey J M

出版信息

Proc Natl Acad Sci U S A. 1987 May;84(9):2771-5. doi: 10.1073/pnas.84.9.2771.

DOI:10.1073/pnas.84.9.2771
PMID:3106977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304740/
Abstract

Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.

摘要

处于第二次分裂期的海胆胚胎已被裂解到微管稳定缓冲液中，以产生有丝分裂细胞骨架（MCS），其由两个被丝状皮质阵列包围的有丝分裂纺锤体组成。通过在0℃下与含钙离子的缓冲液孵育，微管已从MCS中完全提取出来。一种针对微管转运蛋白驱动蛋白的抗体可对MCS以及用5 mM ATP处理的MCS中的纺锤体进行染色，并且在微管被提取后也可对MCS的纺锤体残余物进行染色。我们得出结论，驱动蛋白与有丝分裂纺锤体中的一种非微管成分结合。基于这些结果，我们提出了驱动蛋白在纺锤体中功能的几种模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/f784c5bda60a/pnas00274-0218-f.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/f784c5bda60a/pnas00274-0218-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/ca6d4abfec6c/pnas00274-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/a545f9fbfa8d/pnas00274-0217-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/39fa79ea3318/pnas00274-0217-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/cb5ffb5ff242/pnas00274-0217-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/571399f49399/pnas00274-0217-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/d67da7618507/pnas00274-0217-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/fbb091fdaaa8/pnas00274-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/dbdbd65abbd9/pnas00274-0218-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/d65287d8d6ba/pnas00274-0218-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/24b2ee4eeeac/pnas00274-0218-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/53cf9004ad47/pnas00274-0218-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f873/304740/f784c5bda60a/pnas00274-0218-f.jpg

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An interpretation of transport phenomena at mitosis.有丝分裂时转运现象的一种解释。
Ann N Y Acad Sci. 1960 Oct 7;90:381-408. doi: 10.1111/j.1749-6632.1960.tb23258.x.
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Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).从海胆卵（多色刺海胆）中分离出的对钙敏感的有丝分裂纺锤体。
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Searching for a spindle matrix.寻找纺锤体基质。
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Mitosis, microtubules, and the matrix.有丝分裂、微管与基质。
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Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix.斯克莱托（Skeletor）是一种在有丝分裂期间重新分布的新型染色体蛋白，为纺锤体基质的形成提供了证据。
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Ultraviolet microbeam irradiations of mitotic diatoms: investigation of spindle elongation.有丝分裂硅藻的紫外线微束照射：纺锤体伸长的研究
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Mitotic spindles isolated from sea urchin eggs with EGTA lysis buffers.用乙二醇双四乙酸（EGTA）裂解缓冲液从海胆卵中分离出的有丝分裂纺锤体。
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Interaction of cytoskeletal proteins on the human erythrocyte membrane.细胞骨架蛋白在人红细胞膜上的相互作用。
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Rethinking mitosis.重新审视有丝分裂。
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Fast axonal transport in extruded axoplasm from squid giant axon.鱿鱼巨轴突挤出轴浆中的快速轴突运输。
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