Lee Sumin, Kim Minkyeong, Heo Sojeong, Moon Yura, Lee Gawon, Jeong Do-Won
Department of Food and Nutrition, Dongduk Women's University, Seoul 02748, Republic of Korea.
J Microbiol Biotechnol. 2025 Sep 11;35:e2507004. doi: 10.4014/jmb.2507.07004.
This study aimed to assess the probiotic potential of DMLC16 previously selected as a fermentation starter candidate based on safety and enzymatic activity evaluations by further examining its health-promoting properties and antimicrobial efficacy. Strain DMLC16 exhibited higher acid tolerance, bile salt resistance, and intestinal adhesion ability than its type strain KACC 11860. Moreover, it demonstrated antimicrobial activities against a range of foodborne pathogens and spoilage organisms, including , , , , , sp., and . Strain DMLC16 also showed antifungal activities against , , and . Whole-genome analysis confirmed the safety of DMLC16, revealing the absence of toxin-encoding and plasmids containing acquired antibiotic resistance genes. It also identified genes associated with enzymatic function, salt tolerance, and survival under extreme conditions such as gastric acidity. Although no specific bacteriocin genes were detected, genes implicated in the production of antimicrobial substances were present. The complete genome sequence of DMLC16 provides valuable insight into genetic determinants underlying its probiotic traits and supports its potential application as a functional starter culture or probiotic in the food industry.
本研究旨在通过进一步考察其促进健康的特性和抗菌功效，评估先前基于安全性和酶活性评估被选为发酵起始候选菌株的DMLC16的益生菌潜力。菌株DMLC16比其模式菌株KACC 11860表现出更高的耐酸性、胆盐抗性和肠道黏附能力。此外，它对一系列食源性病原体和腐败菌表现出抗菌活性，包括[具体菌种1]、[具体菌种2]、[具体菌种3]、[具体菌种4]、[具体菌种5]、[具体菌种6]菌属和[具体菌种7]。菌株DMLC16对[具体真菌1]、[具体真菌2]和[具体真菌3]也表现出抗真菌活性。全基因组分析证实了DMLC16的安全性，表明不存在毒素编码基因和含有获得性抗生素抗性基因的质粒。它还鉴定出与酶功能、耐盐性以及在胃酸等极端条件下存活相关的基因。虽然未检测到特定的细菌素基因，但存在与抗菌物质产生相关的基因。DMLC16的完整基因组序列为其益生菌特性背后的遗传决定因素提供了有价值的见解，并支持其作为功能性起始培养物或益生菌在食品工业中的潜在应用。
