氧化铜纳米粒子修饰的还原氧化石墨烯纳米复合材料电化学传感器快速灵敏检测色氨酸
Electrochemical Sensor for Rapid and Sensitive Detection of Tryptophan by a CuO Nanoparticles-Coated Reduced Graphene Oxide Nanocomposite.
机构信息
School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.
Key Laboratory of Functional Metal‒Organic Compounds of Hunan Province; Key Laboratory of Functional Organometallic Materials, University of Hunan Province; Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China.
出版信息
Biomolecules. 2019 May 8;9(5):176. doi: 10.3390/biom9050176.
In this paper, a nanocomposite of cuprous oxide and electrochemically reduced graphene oxide (CuO‒ERGO) was prepared by a simple and low-cost method; hereby, a new method for the electrochemical determination of tryptophan (Trp) by this composite modified glassy carbon electrode (GCE) is proposed. The prepared materials and modified electrodes were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and cyclic voltammetry (CV). The results showed that CuO‒ERGO/GCE had good electrocatalytic activity for Trp. The effects of supporting electrolyte, scanning rate, accumulation potential, and accumulation time on the determination of Trp were studied. Under the optimum experimental conditions, Trp was quantitatively analyzed by square-wave voltammetry (SWV). The oxidation peak current of Trp had a good linear relationship with its concentration in the range of 0.02‒20 μM, and the detection limit was 0.01 μM (S/N = 3). In addition, the modified electrode has high sensitivity, good repeatability, and long-term stability. Finally, the proposed method has been successfully applied in the determination of Trp concentration in practical samples.
本文采用简单、低成本的方法制备了氧化铜和电化学还原氧化石墨烯（CuO-ERGO）纳米复合材料；在此，提出了一种利用该复合材料修饰的玻碳电极（GCE）电化学测定色氨酸（Trp）的新方法。通过扫描电子显微镜（SEM）、X 射线衍射（XRD）和循环伏安法（CV）对制备的材料和修饰电极进行了表征。结果表明，CuO-ERGO/GCE 对 Trp 具有良好的电催化活性。研究了支持电解质、扫描速率、富集电位和富集时间对 Trp 测定的影响。在最佳实验条件下，通过方波伏安法（SWV）对 Trp 进行定量分析。Trp 的氧化峰电流与其浓度在 0.02‒20 μM 范围内呈良好的线性关系，检测限为 0.01 μM（S/N = 3）。此外，修饰电极具有较高的灵敏度、良好的重现性和长期稳定性。最后，该方法已成功应用于实际样品中 Trp 浓度的测定。
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