Department of Physiology, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Cross River State, Nigeria.
Arch Physiol Biochem. 2021 Feb;127(1):51-60. doi: 10.1080/13813455.2019.1610778. Epub 2019 May 10.
Lactate is the preferred energy substrate for developing testicular germ cells. Diabetes is associated with impaired testicular lactate transport/utilisation, and poor sexual behaviour.
To examine the effects of metformin on parameters involved in testicular lactate production, transport/utilisation, and sexual behaviour in diabetic state.
Male rats were assigned into normal control (NC), diabetic control (DC), and metformin-treated diabetic group ( = 6/group). Metformin (300 mg/kg b.w./day) was administrated orally for 4 weeks.
Intra-testicular glucose and lactate levels, and lactate dehydrogenase (LDH) activity increased, while the mRNA transcript levels of genes responsible for testicular glucose and lactate transport/utilisation (glucose transporter 3, monocarboxylate transporter 4 (MCT4), MCT2, and LDH type C) decreased in DC group. Furthermore, penile nitric oxide increased, while cyclic guanosine monophosphate decreased, with impaired sexual behaviour in DC group. Treatment with metformin improved these parameters.
Metformin increases testicular lactate transport/utilisation and improves sexual behaviour in diabetic state.
乳酸是发育中的睾丸生殖细胞的首选能量底物。糖尿病与睾丸乳酸转运/利用受损以及性功能障碍有关。
研究二甲双胍对糖尿病状态下睾丸乳酸生成、转运/利用和性行为相关参数的影响。
雄性大鼠被分为正常对照组（NC）、糖尿病对照组（DC）和二甲双胍治疗糖尿病组（每组 n=6）。二甲双胍（300mg/kg bw/天）经口给药 4 周。
DC 组大鼠睾丸内葡萄糖和乳酸水平以及乳酸脱氢酶（LDH）活性升高，而负责睾丸葡萄糖和乳酸转运/利用的基因（葡萄糖转运蛋白 3、单羧酸转运蛋白 4（MCT4）、MCT2 和 LDH 同工酶 C）的 mRNA 转录水平降低。此外，阴茎一氧化氮增加，环鸟苷酸减少，导致 DC 组的性行为受损。二甲双胍治疗改善了这些参数。
二甲双胍可增加糖尿病状态下睾丸的乳酸转运/利用，并改善性功能。
