使用连续细胞系分离流感病毒。
The use of a continuous cell line for the isolation of influenza viruses.
作者信息
Davies H W, Appleyard G, Cunningham P, Pereira M S
出版信息
Bull World Health Organ. 1978;56(6):991-3.
Abstract
Cultures of MDCK cells exposed to trypsin were as efficient as cultures of rhesus monkey kidney cells for detecting influenza virus, both in dilutions of infected allantoic fluids and in nose and throat swabs. We suggest that the MDCK cell/trypsin system provides a satisfactory alternative to monkey kidney cultures for the isolation of influenza viruses from clinical specimens.
摘要
在检测流感病毒方面，用胰蛋白酶处理过的MDCK细胞培养物与恒河猴肾细胞培养物一样有效，无论是在感染的尿囊液稀释液中，还是在鼻和咽拭子中。我们认为，MDCK细胞/胰蛋白酶系统为从临床标本中分离流感病毒提供了一种令人满意的替代猴肾培养的方法。
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本文引用的文献
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Plaque formation by influenza viruses in the presence of trypsin.流感病毒在胰蛋白酶存在的情况下形成噬菌斑。
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