Wyde P R, Wilson M R, Cate T R
Infect Immun. 1982 Dec;38(3):1249-55. doi: 10.1128/iai.38.3.1249-1255.1982.
Lung fluids and leukocytes were obtained from unprimed C3H mice by transpleural lavage at intervals after infection with influenza A/Hong Kong/68 virus and were tested for interferon activity. Lavage fluid interferon titers correlated directly with lung virus titers and with initial increases in leukocyte yields from infected lungs. In contrast to cultured lymph node cells from infected animals or leukocytes from lungs of uninfected mice, washed leukocytes obtained from the lungs of mice infected 2 to 6 days earlier produced interferon spontaneously in culture. The physiochemical, biological, and antigenic properties of both the interferon in lavage fluids and that produced by lung lavage leukocytes were similar and characteristics of alpha interferon. Fractionation studies indicated that macrophages and T lymphocytes were primarily responsible for the interferon produced in culture. The early presence and significant numbers of interferon-producing leukocytes in infected lungs suggests that these cells have an early role in defense against influenza virus infection.
通过在感染甲型流感病毒/香港/68后不同时间经胸膜腔灌洗，从未经致敏的C3H小鼠获取肺液和白细胞，并检测其干扰素活性。灌洗液中的干扰素滴度与肺病毒滴度以及感染肺中白细胞产量的初始增加直接相关。与感染动物的培养淋巴结细胞或未感染小鼠肺中的白细胞不同，从2至6天前感染的小鼠肺中获得的洗涤白细胞在培养中能自发产生干扰素。灌洗液中的干扰素以及肺灌洗白细胞产生的干扰素的理化、生物学和抗原特性相似，均为α干扰素的特征。分级分离研究表明，巨噬细胞和T淋巴细胞是培养中产生干扰素的主要原因。感染肺中早期出现且数量众多的产生干扰素的白细胞表明，这些细胞在抵抗流感病毒感染的早期防御中发挥作用。
