Moyer C F, Strandberg J D, Reinisch C L
Am J Pathol. 1987 May;127(2):229-42.
The cellular mechanisms governing the expression of mononuclear cell vasculitis are poorly understood. For determination of the precise sequence of events in the development of vasculitis in autoimmune MRL/lpr mice, histologic sections from 4-20-week-old mice were evaluated with a panel of cytochemical and immunohistochemical stains. The results show that vascular disease in MRL/lpr mice develops as follows: Thy 1+, Ly 1+, L3T4- T cells assemble around predominantly small-to-medium muscular arteries at approximately 8 weeks of age. At 12 weeks of age, an adventitial inflammatory focus forms, composed of large "reactive" mononuclear inflammatory cells adjacent to hypertrophied vascular smooth muscle cells (VSMCs). Blastic Thy 1+, Ly 1+, L3T4- T cells subsequently infiltrate the tunica media, and selective VSMC karyolysis results. Occasional cytotoxic/suppressor T cells, macrophages, and possibly NK cells are noted primarily distal to the infiltration site. The outer zone of the inflammatory infiltrate is composed of mature B cells and occasional B-cell precursors. These findings suggest that cellular constituents of the immune response mediate mononuclear cell vasculitis in MRL/lpr mice.
目前对单核细胞性血管炎表达的细胞机制了解甚少。为了确定自身免疫性MRL/lpr小鼠血管炎发展过程中事件的精确顺序，我们用一组细胞化学和免疫组织化学染色方法对4至20周龄小鼠的组织切片进行了评估。结果显示，MRL/lpr小鼠的血管疾病发展如下：在大约8周龄时，Thy 1+、Ly 1+、L3T4 - T细胞主要围绕中小肌性动脉聚集。在12周龄时，形成一个外膜炎症灶，由与肥大的血管平滑肌细胞（VSMC）相邻的大型“反应性”单核炎症细胞组成。随后，母细胞样的Thy 1+、Ly 1+、L3T4 - T细胞浸润中膜，导致选择性的VSMC核溶解。偶尔可见细胞毒性/抑制性T细胞、巨噬细胞，可能还有NK细胞，主要出现在浸润部位的远端。炎症浸润的外层由成熟B细胞和偶尔的B细胞前体组成。这些发现表明，免疫反应的细胞成分介导了MRL/lpr小鼠的单核细胞性血管炎。
