Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Int J Pharm. 2019 Jun 30;565:174-186. doi: 10.1016/j.ijpharm.2019.04.079. Epub 2019 May 7.
Nanotechnology-based platforms have gained a growing interest in skin wound healing. Herein, gold nanoparticles (AuNPs) of different shapes (rods and spheres) and surface modifications (neutral, cationic and anionic charged polymers) were synthesized, characterized and loaded into a thermosensitive hydrogel (poloxamer 407). AuNPs-hydrogels exhibited excellent colloidal stability and demonstrated slow and prolonged release behavior over a 48-h of exposure using in vitro model. Hydrogels of poly ethylene glycol (PEG)-gold nanorods (AuNRs) and cationic poly allyl amine hydrochloride (PAH)-AuNRs demonstrated remarkable wound healing properties upon topical application on wounds using an animal model. PEGylated and cationic charged-AuNRs hydrogels have enhanced skin re-epithelization and collagen deposition after 14 days of daily wound treatment compared to controls, and they affected the gene expression of several inflammatory and anti-inflammatory mediators. Hydrogels of PEG-AuNRs and PAH-AuNRs exhibited potent in vitro antibacterial activity against staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). Furthermore, AuNPs of different shapes and surface modifications demonstrated low percentages of deposition into the main body organs after 21 days of daily wound treatment. Hydrogels of AuNRs could be a promising nano-platform for wound healing.
基于纳米技术的平台在皮肤伤口愈合方面引起了越来越多的关注。在此，合成了不同形状（棒状和球状）和表面修饰（中性、阳离子和阴离子带电聚合物）的金纳米粒子（AuNPs），并对其进行了表征和负载到热敏水凝胶（泊洛沙姆 407）中。AuNPs-水凝胶表现出优异的胶体稳定性，并在体外模型中暴露 48 小时后表现出缓慢且持续的释放行为。使用动物模型在伤口上局部应用时，聚乙二醇（PEG）-金纳米棒（AuNRs）和阳离子聚烯丙基胺盐酸盐（PAH）-AuNRs 的 PEG 化和阳离子电荷-AuNRs 水凝胶表现出显著的伤口愈合特性。与对照组相比，PEG 化和阳离子电荷-AuNRs 水凝胶在每天处理伤口 14 天后，增强了皮肤再上皮化和胶原沉积，并影响了几种炎症和抗炎介质的基因表达。PEG-AuNRs 和 PAH-AuNRs 的水凝胶在体外对金黄色葡萄球菌（S. aureus）和铜绿假单胞菌（P. aeruginosa）表现出很强的抗菌活性。此外，不同形状和表面修饰的 AuNPs 在每天处理伤口 21 天后，进入主要器官的沉积百分比很低。AuNRs 的水凝胶可能是一种有前途的用于伤口愈合的纳米平台。
