State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
Bioresour Technol. 2019 Sep;287:121387. doi: 10.1016/j.biortech.2019.121387. Epub 2019 Apr 29.
Denitrification biofilters (DNBFs) are widely used in advanced nitrogen removal of wastewater with low C/N and effective biofilm formation is critical to their long-term operation. Hereby the influence of rhamnolipid addition in DNBFs was investigated for the first time. Gradient concentrations (0, 20, 40, 80, 120 mg/L) of rhamnolipid were applied to investigate nitrogen removal, biofilm properties and microbial community of lab-scale DNBFs. A significant increase of nitrogen removal was observed in rhamnolipid-treated DNBFs (p < 0.05). Total solid (TS), extracellular polymeric substances (EPS) and adhesion force of biofilms in DNBF with 120 mg/L rhamnolipid reached the maximum, which were 2.17, 2.15 and 3.36 times of those in the control, respectively. Moreover, rhamnolipid exhibited an improvement in abundance of Simplicispira and Gemmatimonas which were responsible for enhanced biofilm formation and denitrification. The results suggested that rhamnolipid addition can be a novel strategy to improve the start-up and denitrification performance of DNBFs.
反硝化生物滤池（DNBFs）广泛应用于低 C/N 废水的深度脱氮，有效生物膜的形成对其长期运行至关重要。本文首次研究了添加鼠李糖脂对 DNBFs 的影响。采用梯度浓度（0、20、40、80、120mg/L）的鼠李糖脂来考察实验室规模的 DNBFs 的脱氮效果、生物膜特性和微生物群落。结果表明，添加鼠李糖脂后 DNBFs 的脱氮效果显著提高（p<0.05）。在添加 120mg/L 鼠李糖脂的 DNBF 中，总固体（TS）、胞外聚合物（EPS）和生物膜的附着力分别达到了对照组的 2.17、2.15 和 3.36 倍。此外，鼠李糖脂的添加提高了固氮螺菌属和芽单胞菌属的丰度，这两种菌属负责增强生物膜的形成和反硝化作用。研究结果表明，添加鼠李糖脂可以作为一种提高 DNBFs 启动和反硝化性能的新策略。
