Key Laboratory of Algal Biology of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Algal Biology of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Bioresour Technol. 2020 May;303:122905. doi: 10.1016/j.biortech.2020.122905. Epub 2020 Jan 28.
A strain with efficient biofilm-formation and aerobic denitrification capabilities was isolated and identified as Pseudomonas mendocina IHB602. In pure culture, strain IHB602 removed almost all NO-N, NO-N, and NH-N (initial concentrations 50 mg/L) within 24 h. The strain produced large amounts of extracellular polymeric substances (maximum 430.33 mg/g cell dry weight) rich in protein but containing almost no humic acid. This, and strong autoaggregation (maximum 47.09%) and hydrophobicity (maximum 85.07%), imparted strain IHB602 with biofilm forming traits. A sequencing batch biofilm reactor bioaugmented with strain IHB602 (SBBR1) had more rapid biofilm-formation than the control without strain IHB602 inoculation (SBBR2). During the stabilization period, the effluent removal ratios for NH-N (95%), NO-N (91%) and TN (88%) in SBBR1 were significantly higher than those in SBBR2 (NH-N: 91%, NO-N: 88%, TN: 82%). Microbial community structure analysis revealed that strain IHB602 successfully proliferated and contributed to nitrogen removal as well as biofilm formation.
一株具有高效生物膜形成和有氧反硝化能力的菌株被分离并鉴定为门多萨假单胞菌 IHB602。在纯培养条件下，菌株 IHB602 能够在 24 小时内去除几乎所有的硝态氮（初始浓度 50mg/L）、亚硝态氮和氨氮。该菌株产生大量的胞外聚合物（最大 430.33mg/g 细胞干重），这些聚合物富含蛋白质，但几乎不含腐殖酸。这使得菌株 IHB602 具有较强的自动聚集能力（最大 47.09%）和疏水性（最大 85.07%），从而具有生物膜形成特性。用菌株 IHB602 强化的序批式生物膜反应器（SBBR1）比未接种菌株 IHB602 的对照反应器（SBBR2）具有更快的生物膜形成速度。在稳定期，SBBR1 对 NH-N（95%）、NO-N（91%）和 TN（88%）的去除率明显高于 SBBR2（NH-N：91%，NO-N：88%，TN：82%）。微生物群落结构分析表明，菌株 IHB602 成功增殖，并有助于氮的去除和生物膜的形成。
