Chang Siyuan, He Xuejun, Li Bingfeng, Pan Xin
School of Biology and Environment, Nanjing Polytechnic Institute, Nanjing, China.
Department of Cardiology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Front Chem. 2021 Feb 3;9:635191. doi: 10.3389/fchem.2021.635191. eCollection 2021.
Upgrading of biomass derived 5-hydroxymethylfurfural (HMF) has attracted considerable interest recently. A new highly HMF-tolerant strain of NJPI-15 was isolated in this study, and the biocatalytic reduction of HMF into 2,5-bis(hydroxymethyl)furan (BHMF) using whole cells was reported. Co-substrate was applied to improve the BHMF yield and selectivity of this strain as well as HMF-tolerant level. The catalytic capacity of the cells can be substantially improved by Mn ion. The strain exhibited good catalytic performance at a pH range of 6.0-9.0 and a temperature range of 25°C-35°C. In addition, 100 mM HMF could be reduced to BHMF by the NJPI-15 resting cells in presence of 70 mM glutamine and 30 mM sucrose, with a yield of 95%. In the fed-batch strategy, 656 mM BHMF was obtained within 48 h, giving a yield of 93.7%. The reported utilization of HMF to produce BHMF is a promising industrially sound biocatalytic process.
生物质衍生的5-羟甲基糠醛（HMF）的升级改造近来引起了广泛关注。本研究分离出一种新型的高耐HMF菌株NJPI-15，并报道了使用全细胞将HMF生物催化还原为2,5-双（羟甲基）呋喃（BHMF）的过程。应用共底物来提高该菌株的BHMF产量、选择性以及耐HMF水平。锰离子可大幅提高细胞的催化能力。该菌株在pH值6.0 - 9.0和温度25°C - 35°C范围内表现出良好的催化性能。此外，在70 mM谷氨酰胺和30 mM蔗糖存在的情况下，NJPI-15静止细胞可将100 mM HMF还原为BHMF，产率为95%。在分批补料策略中，48小时内可获得656 mM BHMF，产率为93.7%。所报道的利用HMF生产BHMF的方法是一种具有工业应用前景的生物催化过程。
