Department for Life Quality Studies, University of Bologna, Rimini, Italy.
Department of Life Sciences and Biotechnology, University of Ferrara, Malborghetto di Boara, Ferrara, Italy.
Sci Rep. 2019 May 10;9(1):7199. doi: 10.1038/s41598-019-43609-5.
Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus's phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area.
蛋白酶体抑制代表了一种重要的抗癌策略。在这里，我们研究了导致具有复杂抗癌活性的植物印度青黛标准化煎剂促凋亡活性的基础机制，并探讨了其对人白血病细胞中蛋白酶体活性的抑制作用。此外，我们初步测试了印度青黛的一些植物化学物质对白血病细胞的细胞毒性及其在由分化的 Caco2 细胞单层组成的人肠道上皮模型中的肠道吸收。我们观察到印度青黛具有很强的抗白血病作用，这归因于不同关键靶点在蛋白质和 mRNA 水平上的调节以及 26S 蛋白酶体表达的减少。我们发现印度青黛煎剂的一些植物标志物穿过肠细胞层。总的来说，我们的研究支持了印度青黛的药理学潜力，它可以成为肿瘤学领域中一种有趣的植物药物。
