Univ. Grenoble Alpes, Inserm 1042, CHU Grenoble Alpes, HP2 Laboratory, Grenoble, France.
Cardiovascular Research Laboratory, Spaulding Rehabilitation Hospital, Cambridge, Massachusetts, USA.
Sci Rep. 2019 May 10;9(1):7252. doi: 10.1038/s41598-019-43696-4.
Muscle wasting reduces functional capacity and increases cardiometabolic risk in chronic disease. Neuromuscular electrical stimulation (NMES) of the lower limb has been shown to reverse muscle wasting in these patients but its effect on cardiometabolic health is unclear. We investigated a mouse model of in-vivo non-invasive chronic NMES on muscle mass, insulin sensitivity and arterial blood pressure (BP). Twenty-three C57BL6 mice underwent unilateral NMES or sham training over 2.5 weeks while anesthetized by isoflurane. Lower limb muscle mass and the stimulated limb to non-stimulated limb muscle mass ratio were compared between groups (NMES vs. sham). Insulin sensitivity was assessed 48 h after training using an intraperitoneal insulin tolerance test (ITT) and BP was assessed before and after training using the tail-cuff technique. After training, muscle mass increased in NMES vs. sham (416 ± 6 vs. 397 ± 6 mg, p = 0.04) along with the ratio of muscle mass (+3 ± 1% vs. -1 ± 1% p = 0.04). Moreover, insulin sensitivity improved in NMES vs. sham (average blood glucose during ITT: 139.6 ± 8.5 vs. 161.9 ± 9.0 mg/dl blood, p = 0.01). BP was decreased in both groups, although it is likely that the effect of NMES on BP was dampened by repetitive anesthesia. The metabolic benefit of NMES training could be of great utility in patients with chronic disease. Moreover, the clinical-like mouse model of NMES is an effective tool to investigate the systemic effects of local muscle strengthening.
肌肉减少症降低了慢性疾病患者的功能能力，并增加了其患心血管代谢疾病的风险。下肢神经肌肉电刺激（NMES）已被证明可逆转这些患者的肌肉减少症，但它对心血管代谢健康的影响尚不清楚。我们研究了一种在体无创慢性 NMES 对肌肉质量、胰岛素敏感性和动脉血压（BP）的小鼠模型。23 只 C57BL6 小鼠在异氟烷麻醉下接受单侧 NMES 或假训练 2.5 周。在组间比较（NMES 与假训练）比较下肢肌肉质量和受刺激肢体与未受刺激肢体的肌肉质量比。在训练后 48 小时使用腹腔内胰岛素耐量试验（ITT）评估胰岛素敏感性，并用尾套技术在训练前后评估 BP。在训练后，NMES 组的肌肉质量增加（416±6 与 397±6mg，p=0.04），肌肉质量比增加（+3±1%与-1±1%，p=0.04）。此外，NMES 组的胰岛素敏感性改善（ITT 期间平均血糖：139.6±8.5 与 161.9±9.0mg/dl 血，p=0.01）。尽管 NMES 对 BP 的影响可能因重复麻醉而减弱，但两组的 BP 均降低。NMES 训练的代谢益处可能对慢性疾病患者非常有用。此外，NMES 的临床样小鼠模型是研究局部肌肉增强的全身效应的有效工具。
