Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
The Ohio State University Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA.
Nucleic Acids Res. 2019 Jun 20;47(11):5490-5501. doi: 10.1093/nar/gkz295.
RNA is an essential player in almost all biological processes, and has an ever-growing number of roles in regulating cellular growth and organization. RNA functions extend far beyond just coding for proteins and RNA has been shown to function in signaling events, chromatin organization and transcriptional regulation. Dissecting how the complex network of RNA-binding proteins (RBPs) and regulatory RNAs interact with their substrates within the cell is a real, but exciting, challenge for the RNA community. Investigating these biological questions has fueled the development of new quantitative technologies to measure how RNA and RBPs interact both locally and on a global scale. In this review, we provide an assessment of available approaches to enable researchers to select the protocol most applicable for their experimental question.
RNA 是几乎所有生物过程中的重要参与者，并且在调节细胞生长和组织方面具有越来越多的作用。RNA 的功能远远超出了编码蛋白质的功能，并且已经证明 RNA 在信号事件、染色质组织和转录调控中发挥作用。解析 RNA 结合蛋白 (RBP) 和调节 RNA 与其在细胞内的底物之间的复杂网络如何相互作用，对于 RNA 领域来说是一个真实而令人兴奋的挑战。研究这些生物学问题推动了新的定量技术的发展，以衡量 RNA 和 RBP 如何在局部和全局范围内相互作用。在这篇综述中，我们评估了可用的方法，以使研究人员能够选择最适用于他们实验问题的方案。
