Laboratorio de Biotecnología de los Alimentos, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.
Doctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Universidad Católica del Norte, Pontificia Universidad Católica de Valparaíso, Santiago, Chile.
Probiotics Antimicrob Proteins. 2020 Jun;12(2):672-682. doi: 10.1007/s12602-019-09550-9.
Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortality. Intestinal microbiota are involved in many processes that benefit a host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism. The aim of this study is to evaluate the in vitro probiotic properties of bacteria isolated from the intestinal content of wild Seriola lalandi. The probiotic potential was evaluated in terms of (i) the antimicrobial activity against vibrios causing outbreaks in farmed fish; (ii) the ability to stimulate genes related to an innate immune response in fish; and (iii) antibiotic resistance. Nineteen isolates identified as Pseudomonas, Shewanella, Psychrobacter, and Acinetobacter showed antimicrobial activity and significant relative expression of cytokines, serum amyloid A protein (SAA), hepcidin, and lysozyme. A positive correlation was observed between the levels of expression and the bacterial load after 24 h of exposure. Pseudomonas isolates showed a level of antibiotic resistance. In conclusion, isolates of the genera Shewanella, Psychrobacter, and Acinetobacter could serve as potential probiotics in S. lalandi culture.
蓝圆鲹（Seriola lalandi）是一种具有重要经济价值的物种，在全球温带和亚热带海洋水域均有分布。该物种的水产养殖生产存在与集约化养鱼相关的问题，例如疾病爆发或营养缺乏导致高死亡率。肠道微生物群参与许多有益于宿主的过程，例如疾病控制、免疫反应刺激和促进营养代谢。本研究旨在评估从野生蓝圆鲹肠道内容物中分离出的细菌的体外益生菌特性。益生菌潜力是通过以下几个方面来评估的：（i）对引起养殖鱼类疾病爆发的弧菌的抗菌活性；（ii）刺激鱼类先天免疫反应相关基因的能力；和（iii）抗生素耐药性。19 株被鉴定为假单胞菌、希瓦氏菌、嗜冷杆菌和不动杆菌的分离株表现出抗菌活性和细胞因子、血清淀粉样蛋白 A 蛋白 (SAA)、铁调素和溶菌酶的显著相对表达。在暴露 24 小时后，观察到表达水平与细菌负荷之间存在正相关。假单胞菌分离株表现出一定水平的抗生素耐药性。综上所述，希瓦氏菌、嗜冷杆菌和不动杆菌属的分离株可作为蓝圆鲹养殖中的潜在益生菌。
