Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
Nutrients. 2020 Dec 27;13(1):55. doi: 10.3390/nu13010055.
Allergy can cause intestinal damage, including through cell apoptosis. In this study, intestinal cell apoptosis was first observed in the β-conglycinin (β-CG) allergy model, and the effect of GG (LGG) on reducing apoptosis of cells in the intestine and its underlying mechanisms were further investigated. Allergic mice received oral LGG daily, and intestinal tissue apoptotic cells, gut microbiota, and metabolites were evaluated six and nine days after intervention. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analysis revealed that LGG intervention could reduce the incidence of cell apoptosis more effectively than natural recovery (NR). The results of 16S rRNA analysis indicated that LGG intervention led to an increase in the relative abundance of . Metabolite analysis of intestinal contents indicated that histamine, -acetylhistamine, (α)-γ-glutamylhistamine, phenylalanine, tryptophan, arachidonic acid malate, and xanthine were significantly decreased, and deoxycholic acid, lithocholic acid were significantly increased after the LGG intervention on β-CG allergy; the decreases in histamine and (α)-γ-glutamylhistamine were significant compared with those of NR. In conclusion, LGG reduces apoptosis of cells induced by β-CG allergy, which may be related to regulation of and the bile secretion pathway.
过敏会导致肠道损伤，包括通过细胞凋亡。在这项研究中，首次观察到β-伴大豆球蛋白（β-CG）过敏模型中的肠细胞凋亡，进一步研究了 GG（LGG）减少肠道细胞凋亡的作用及其潜在机制。过敏小鼠每天口服 LGG，干预后 6 天和 9 天评估肠道组织凋亡细胞、肠道微生物群和代谢物。末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记（TUNEL）分析表明，LGG 干预比自然恢复（NR）更有效地减少细胞凋亡的发生率。16S rRNA 分析结果表明，LGG 干预导致相对丰度的增加。肠道内容物代谢物分析表明，β-CG 过敏后，LGG 干预可显著降低组氨酸、-乙酰组氨酸、（α）-γ-谷氨酰组氨酸、苯丙氨酸、色氨酸、花生四烯酸马来酸和黄嘌呤的含量，而去氧胆酸、石胆酸的含量显著增加；与 NR 相比，组氨酸和（α）-γ-谷氨酰组氨酸的减少更为显著。总之，LGG 减少了 β-CG 过敏引起的细胞凋亡，这可能与调节和胆汁分泌途径有关。
