Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Sakha Aquaculture Research Unit, Kafrelsheikh, Egypt.
Probiotics Antimicrob Proteins. 2020 Jun;12(2):389-399. doi: 10.1007/s12602-019-09552-7.
The current study was conducted to evaluate the synergetic effects of heat-killed Lactobacillus plantarum (HK L-137) and β-glucan (BG) on digestive enzyme activity and intestinal morphology of genetically improved farmed tilapia (GIFT) with focus on insulin-like growth factor I (IGF-I), fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G6PD). For 12 weeks, fish fed the control, or three diets incorporated with 100 HK L-137, 100 BG, or 50 HK L-137 + 50 BG mg/kg (HK L-137, BG, and HK L-137/BG diets). After final sampling, fish fed HK L-137 or HK L-137/BG diets exhibited significantly (P < 0.05) increased final body weight and weight gain while the specific growth rate and feed efficiency ratio enhanced only in HK L-137/BG group. Mucosal and villi lengths and muscle thickness significantly (P < 0.05) increased by HK L-137 or/and BG for the middle intestine. Lipase and protease improved significantly (P < 0.05) in fish fed both HK L-137 and BG when compared to the control group. Interestingly, qRT-PCR revealed a significant (P < 0.05) upregulation in the IGF-1 gene expression in fish fed HK L-137 or/and BG additives compared to the control. Muscle and liver G6PD gene expression were upregulated significantly (P < 0.05) in fish fed HK L-137/BG diet as compared to the control group. In addition, feeding HK L-137 or both additives effectively elevated the hematocrit, hemoglobin, and WBCs and decreased triglyceride and glucose levels. Accordingly, the use of both HK L-137 and BG is an efficient scheme to reach economically feasible and sustainable tilapia production.
本研究旨在评估热灭活植物乳杆菌（HK L-137）和β-葡聚糖（BG）对遗传改良农场罗非鱼（GIFT）消化酶活性和肠道形态的协同作用，重点关注胰岛素样生长因子 I（IGF-I）、脂肪酸合成酶（FAS）和葡萄糖-6-磷酸脱氢酶（G6PD）。在 12 周的时间里，鱼分别喂食对照饲料或三种饲料，分别含有 100 HK L-137、100 BG 或 50 HK L-137+50 BG mg/kg（HK L-137、BG 和 HK L-137/BG 饲料）。最后一次采样后，喂食 HK L-137 或 HK L-137/BG 饲料的鱼的终体重和增重显著增加（P<0.05），而特定生长率和饲料效率比仅在 HK L-137/BG 组中提高。HK L-137 或/和 BG 使中肠的粘膜和绒毛长度以及肌肉厚度显著增加（P<0.05）。与对照组相比，喂食 HK L-137 和 BG 的鱼的脂肪酶和蛋白酶活性显著提高（P<0.05）。有趣的是，与对照组相比，喂食 HK L-137 或/和 BG 添加剂的鱼的 IGF-1 基因表达显著上调（P<0.05）。与对照组相比，喂食 HK L-137/BG 饲料的鱼的肌肉和肝脏 G6PD 基因表达显著上调（P<0.05）。此外，喂食 HK L-137 或两种添加剂均可有效提高红细胞压积、血红蛋白和白细胞计数，并降低甘油三酯和葡萄糖水平。因此，同时使用 HK L-137 和 BG 是一种经济可行且可持续的罗非鱼生产方案。
