质谱流式细胞术试剂的滴定

Titration of Mass Cytometry Reagents.

作者信息

van Vreden Caryn, Niewold Paula, McGuire Helen M, Fazekas de St Groth Barbara

机构信息

Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia.

Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, Australia.

出版信息

Methods Mol Biol. 2019;1989:83-92. doi: 10.1007/978-1-4939-9454-0_6.

DOI:10.1007/978-1-4939-9454-0_6

PMID:31077100

Abstract

Mass cytometry (MC) is a powerful research tool enabling high-dimensional analysis of single cells in suspension and within tissue sections following laser ablation. Here we describe the procedure of titrating metal-conjugated antibodies, to ensure that optimal levels of staining are achieved while minimizing nonspecific signals that may occur at high concentrations.

摘要

质谱流式细胞术（MC）是一种强大的研究工具，能够对悬浮液中的单细胞以及激光消融后的组织切片进行高维分析。在此，我们描述了滴定金属偶联抗体的步骤，以确保在将高浓度时可能出现的非特异性信号降至最低的同时，实现最佳的染色水平。