Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Department of Biology, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Department of Biology, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
Int J Biol Macromol. 2019 Aug 1;134:255-261. doi: 10.1016/j.ijbiomac.2019.05.055. Epub 2019 May 9.
A complete absence of the physicochemical characterization of Gracilaria chouae polysaccharides (GCP) and its corresponding bioactivities urged the need for this study. It was found that GCP is a heteropolysaccharide which exists in linear random coil conformation. It contained a sulfate content of 7.9% in addition to 52.63% total sugar content and 9.62% galacturonic acid. Galactose and 3,6-anhydrogalactose were found in a molar ratio of 1.0:0.6. Its setting and melting points were determined as 41.3 and 71.7 °C, respectively, which makes it a suitable candidate for industrial processing where further heating is required and/or where the end-product needs to have extended shelf life in hot climate. GCP demonstrated 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis and hydroxyl radical scavenging activity (36.86, 27.42 and 19.07% at 3 mg·ml). Moreover, the results also suggested its potential use as a prebiotic due to its perceived high fermentability.
由于缺乏对秀丽鹿角菜多糖（GCP）的理化特性及其相应生物活性的全面研究，因此有必要进行本研究。研究发现，GCP 是一种杂多糖，以线性无规卷曲构象存在。它除了含有 52.63%的总糖含量和 9.62%的半乳糖醛酸外，还含有 7.9%的硫酸根。半乳糖和 3,6-脱水半乳糖的摩尔比为 1.0:0.6。其凝固点和熔点分别为 41.3°C 和 71.7°C，使其成为适合工业加工的候选物，因为在工业加工中需要进一步加热和/或需要在炎热气候下延长最终产品的保质期。GCP 表现出 2,2-二苯基-1-苦基肼、2,2'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）和羟基自由基清除活性（在 3mg·ml 时分别为 36.86%、27.42%和 19.07%）。此外，由于其高发酵性，研究结果还表明其具有作为益生元的潜力。
