黑腹果蝇蛋白激酶C基因的结构与核苷酸序列
Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.
作者信息
Rosenthal A, Rhee L, Yadegari R, Paro R, Ullrich A, Goeddel D V
出版信息
EMBO J. 1987 Feb;6(2):433-41. doi: 10.1002/j.1460-2075.1987.tb04773.x.
Genomic and cDNA clones encoding a Drosophila melanogaster protein kinase C (PKC) homologue were identified using a bovine PKC cDNA probe. The cDNA clones contain a single open reading frame that encodes a 639 amino acid, 75-kd protein having extensive homology with bovine, human and rat PKC and homology with the kinase domains of other serine, threonine and tyrosine kinases. The Drosophila PKC gene is localized to region 53E of chromosome 2. The gene spans approximately 20 kb and contains at least 14 exons. Messenger RNA for PKC could not be detected in 0-3 h Drosophila embryos. Adult flies contain three PKC transcripts of 4.3, 4.0 and 2.4 kb.
使用牛蛋白激酶C（PKC）cDNA探针鉴定出了编码黑腹果蝇蛋白激酶C（PKC）同源物的基因组克隆和cDNA克隆。这些cDNA克隆包含一个单一的开放阅读框，其编码一个639个氨基酸、75千道尔顿的蛋白质，该蛋白质与牛、人和大鼠的PKC具有广泛的同源性，并且与其他丝氨酸、苏氨酸和酪氨酸激酶的激酶结构域具有同源性。果蝇PKC基因定位于2号染色体的53E区域。该基因跨度约20kb，包含至少14个外显子。在0 - 3小时的果蝇胚胎中未检测到PKC的信使RNA。成年果蝇含有4.3、4.0和2.4kb的三种PKC转录本。
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