Macphee C H, Drummond A H, Otto A M, Jimenez de Asua L
J Cell Physiol. 1984 Apr;119(1):35-40. doi: 10.1002/jcp.1041190107.
Prostaglandin F2 alpha (PGF2 alpha); which stimulates DNA synthesis in resting 3T3 cells, also stimulates the incorporation of [32P]PO4 into phosphatidylinositol. The effect is selective for PGF2 alpha when compared with PGE1, PGE2, and PGF2 beta. Epidermal growth factor (EGF) also stimulates DNA synthesis but does not affect phosphatidylinositol turnover. PGE1, which acts synergistically with PGF2 alpha to enhance DNA synthesis, does not affect the ability of PGF2 alpha, to enhance the incorporation of [32P]PO4 into phosphatidylinositol. PGF2 alpha, also causes a small increase in the cellular content of 1,2-diacylglycerol. This effect is not shared by EGF or PGE1. Stimulation of phosphatidylinositol metabolism resulting in an increase in the cellular content of 1,2-diacylglycerol may thus constitute an event in the pathway leading to the initiation of DNA synthesis in which PGF2 alpha differs in its action from EGF.
前列腺素F2α（PGF2α）可刺激静止的3T3细胞中的DNA合成，同时也能刺激[32P]PO4掺入磷脂酰肌醇。与前列腺素E1（PGE1）、前列腺素E2（PGE2）和前列腺素F2β（PGF2β）相比，该作用对PGF2α具有选择性。表皮生长因子（EGF）也能刺激DNA合成，但不影响磷脂酰肌醇的周转。PGE1与PGF2α协同作用增强DNA合成，但不影响PGF2α增强[32P]PO4掺入磷脂酰肌醇的能力。PGF2α还会使细胞内1,2 - 二酰甘油的含量略有增加。EGF或PGE1则没有这种作用。因此，刺激磷脂酰肌醇代谢导致细胞内1,2 - 二酰甘油含量增加，可能是导致DNA合成起始途径中的一个事件，在这一途径中PGF2α的作用与EGF不同。
