B淋巴细胞增殖反应的抑制机制：免疫复合物对抗μ抗体和B细胞刺激因子1诱导的增殖的抑制作用。
Inhibitory mechanism of the proliferative response of B lymphocytes: suppression of the proliferation induced by anti-mu antibody and BSF1 by immune complexes.
作者信息
Miyama-Inaba M, Ohno T, Inaba K, Ajisaka K, Suzuki R, Kumagai K, Masuda T
出版信息
Immunology. 1987 May;61(1):43-50.
We studied the effect of immune complexes (IC) on the responses of polyclonally activated murine B cells. For this, normal resting B cells were stimulated with the F(ab')2 fraction of goat anti-mouse mu-chain antibody and B-cell stimulating factor 1 (BSF1) after preculturing them with IC. Next, the relative membrane potential changes and the subsequent proliferative response were analysed. IC, particularly in antibody excess, inhibited both membrane depolarization and while those in antigen excess did poorly. Neither antigen nor antibody alone was effective. Inhibition was mediated via binding of IC to FcR gamma on B cells in a dose-dependent manner. Kinetic experiments showed that at least 6 hr was necessary for inducing the suppression of B-cell responses after binding of IC. We conclude that IC bound to FcR gamma on B cells regulates B-cell responses by acting on the initial step of activation.
我们研究了免疫复合物（IC）对多克隆激活的小鼠B细胞反应的影响。为此，在用IC预培养正常静息B细胞后，用山羊抗小鼠μ链抗体的F(ab')2片段和B细胞刺激因子1（BSF1）刺激它们。接下来，分析相对膜电位变化和随后的增殖反应。IC，特别是在抗体过量时，抑制膜去极化，而抗原过量时则效果不佳。单独的抗原或抗体均无效。抑制作用是通过IC以剂量依赖的方式与B细胞上的FcRγ结合介导的。动力学实验表明，IC结合后至少需要6小时才能诱导B细胞反应的抑制。我们得出结论，结合在B细胞上FcRγ的IC通过作用于激活的初始步骤来调节B细胞反应。
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