Fossati G, Anichini A, Parmiani G
Int J Cancer. 1987 Jun 15;39(6):689-94. doi: 10.1002/ijc.2910390606.
Three lymphocyte clones, derived by micromanipulation from peripheral blood lymphocytes (PBL) of a melanoma patient and expressing a broad pattern of reactivity against different target cells, were analyzed for the involvement of T-cell markers and HLA antigens in the lysis of target cells by blocking experiments with a panel of monoclonal antibodies (MAbs). The clones lysed autologous melanoma cells (Me 28) and 18 out of 22 allogeneic targets including neoplastic and normal cells of different histological origin. Anti-T3 and anti-T8 MAbs strongly inhibited the cytotoxicity of the lymphocyte clones against Me 28, 3 allogeneic melanomas and 3 carcinomas, but failed to affect the lysis of K562. Anti-HLA class-I MAb (w6/32) produced a significant enhancement of the lysis of Me 28 by the 3 clones without modifying cytotoxicity against one allogeneic melanoma or against K562 cells. Anti-HLA class-II MAb (D1.12) did not affect the lysis of the same targets by the 3 clones. These results thus indicate that some anti-melanoma CTL clones may interact with autologous tumor cells by the T3 and T8 structures in an HLA class-I unrestricted manner.
从一名黑色素瘤患者的外周血淋巴细胞（PBL）通过显微操作获得了三个淋巴细胞克隆，它们对不同靶细胞表现出广泛的反应模式。通过用一组单克隆抗体（MAb）进行阻断实验，分析了T细胞标志物和HLA抗原在靶细胞裂解中的作用。这些克隆裂解自体黑色素瘤细胞（Me 28）以及22个异基因靶标中的18个，包括不同组织学来源的肿瘤细胞和正常细胞。抗T3和抗T8单克隆抗体强烈抑制淋巴细胞克隆对Me 28、3种异基因黑色素瘤和3种癌的细胞毒性，但不影响对K562的裂解。抗HLA I类单克隆抗体（w6/32）显著增强了这3个克隆对Me 28的裂解，而不改变对一种异基因黑色素瘤或K562细胞的细胞毒性。抗HLA II类单克隆抗体（D1.12）不影响这3个克隆对相同靶标的裂解。因此，这些结果表明，一些抗黑色素瘤CTL克隆可能通过T3和T8结构以HLA I类非限制性方式与自体肿瘤细胞相互作用。
